Document Detail

FOXO1 Expression and Regulation in Endometrial Tissue during the Menstrual Cycle and in Early Pregnancy Decidua.
MedLine Citation:
PMID:  22538365     Owner:  NLM     Status:  Publisher    
Aims: To determine FOXO1 (forkhead box O1) expression in the human endometrium during the menstrual cycle and decidua of early pregnancy, as well as FOXO1 regulation in endometrial stromal cells (ESC). Methods: Immunohistochemistry, RT-qPCR, and Western blot analyses evaluated cellular localization and altered FOXO1 expression in the endometrium during the menstrual cycle and decidua of early pregnancy (proliferative phase, n = 12; early-secretory phase, n = 7; mid-secretory phase, n = 10; late-secretory phase, n = 10; early pregnancy, n = 12). Using RT-qPCR and Western blot, we studied the regulation of FOXO1 by 8-bromo-cAMP, 4-pregnene-3,20-dione, 17β-estradiol, and human chorionic gonadotrophin in ESC (n = 5). Results: The expression level of FOXO1 in human endometrial tissue fluctuated with the menstrual cycle. If pregnancy occurred, the expression of FOXO1 was further increased (p < 0.05) and cAMP regulated FOXO1 expression in ESC. In addition, 4-pregnene-3,20-dione cooperatively stimulated FOXO1 expression with cAMP. We also observed FOXO1 expression during in vitro cAMP-induced decidualization. Conclusions: The pattern of FOXO1 expression suggests a potential role for FOXO1 in implantation and decidualization.
Wei Fan; Shang-Wei Li; Wei-Hong Li; Yan Wang; Yan Gong; Qian-Hong Ma; San Luo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-24
Journal Detail:
Title:  Gynecologic and obstetric investigation     Volume:  -     ISSN:  1423-002X     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7900587     Medline TA:  Gynecol Obstet Invest     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Department of Obstetrics and Gynecology, Center of Reproductive Medicine, The West China Second University Hospital, Sichuan University, Sichuan, PR China.
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