Document Detail


FOXL2 is regulated during the bovine estrous cycle and its expression in the endometrium is independent of conceptus-derived interferon tau.
MedLine Citation:
PMID:  22623620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
FOXL2, a winged-helix/forkhead domain transcription factor, is a key gene involved in the differentiation and biological functions of the ovary. In a recent transcriptomic analysis, we found that FOXL2 expression in bovine caruncular endometrium was different from that in intercaruncular endometrium. In order to gain new insights into FOXL2 in this tissue, we determined the expression of this transcription factor during the estrous cycle and the establishment of pregnancy in cattle. The endometrial expression of FOXL2 did not vary during maternal recognition of pregnancy (Days 16-20). Using an in vivo bovine model and primary cell cultures, we showed that FOXL2 was not an interferon-tau target gene. Both FOXL2 transcript and protein were expressed from Day 5 to Day 20 of the estrous cycle, and their levels showed a significant increase during the luteolytic phase. A 2-day progesterone supplementation in heifers led to a clear down-regulation of FOXL2 protein levels, suggesting the negative impact of progesterone on FOXL2 expression. Immunohistochemistry data revealed the localization of FOXL2 in endometrial stromal and glandular cells. FOXL2 subcellular distribution was shown to be nuclear in endometrial samples collected during the luteolytic period, while it was not detected in nuclei during the luteal phase and at implantation. Collectively, our findings provide the first evidence that FOXL2 is involved in the regulation of endometrial tissue physiology.
Authors:
Caroline Eozenou; Anaïs Vitorino Carvalho; Anaïs Vitorino Carvalho; Niamh Forde; Corinne Giraud-Delville; Laurence Gall; Pat Lonergan; Aurélie Auguste; Gilles Charpigny; Christophe Richard; Maëlle Pannetier; Olivier Sandra
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-09
Journal Detail:
Title:  Biology of reproduction     Volume:  87     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-10     Completed Date:  2013-01-02     Revised Date:  2013-01-23    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  32     Citation Subset:  IM    
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / physiology*
Embryo Implantation
Endometrium / metabolism*
Estrous Cycle*
Female
Forkhead Transcription Factors / metabolism*
Interferon Type I / physiology
Pregnancy
Pregnancy Proteins / physiology
Progesterone / pharmacology
Chemical
Reg. No./Substance:
0/Forkhead Transcription Factors; 0/Interferon Type I; 0/Pregnancy Proteins; 0/trophoblastin; 57-83-0/Progesterone
Comments/Corrections
Erratum In:
Biol Reprod. 2012 Oct;87(4):102
Note: Carvalho, Anaïs Vitorino [corrected to Vitorino Carvalho, Anaïs]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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