| FMT-XCT: in vivo animal studies with hybrid fluorescence molecular tomography-X-ray computed tomography. | |
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MedLine Citation:
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PMID: 22561987 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The development of hybrid optical tomography methods to improve imaging performance has been suggested over a decade ago and has been experimentally demonstrated in animals and humans. Here we examined in vivo performance of a camera-based hybrid fluorescence molecular tomography (FMT) system for 360° imaging combined with X-ray computed tomography (XCT). Offering an accurately co-registered, information-rich hybrid data set, FMT-XCT has new imaging possibilities compared to stand-alone FMT and XCT. We applied FMT-XCT to a subcutaneous 4T1 tumor mouse model, an Aga2 osteogenesis imperfecta model and a Kras lung cancer mouse model, using XCT information during FMT inversion. We validated in vivo imaging results against post-mortem planar fluorescence images of cryoslices and histology data. Besides offering concurrent anatomical and functional information, FMT-XCT resulted in the most accurate FMT performance to date. These findings indicate that addition of FMT optics into the XCT gantry may be a potent upgrade for small-animal XCT systems. |
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Authors:
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Angelique Ale; Vladimir Ermolayev; Eva Herzog; Christian Cohrs; Martin Hrabé de Angelis; Vasilis Ntziachristos |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-06 |
Journal Detail:
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Title: Nature methods Volume: - ISSN: 1548-7105 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101215604 Medline TA: Nat Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Technische Universität München and Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Neuherberg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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