Document Detail

FLICE-inhibitory protein: expression in early and late gestation human placentas.
MedLine Citation:
PMID:  16174530     Owner:  NLM     Status:  MEDLINE    
The apoptosis cascade that plays a central role in normal and pathological processes is strictly controlled, in part by FLIP (Fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein), an inhibitor of caspase-8. Here, we report the expression of long and short isoforms of FLIP mRNAs and proteins in early and late gestation human placentas, term cytotrophoblast cells and two choriocarcinoma cell lines, JEG-3 and Jar. Reverse transcriptase polymerase chain reaction identified mRNAs derived from the FLIP gene in all samples. Analysis by immunoblotting revealed that both long and short forms of FLIP proteins are present in early and late gestation human placentas with increasing levels over gestation and that FLIP proteins are present in normal and transformed trophoblast cells. Immunohistochemical experiments performed on paraformaldehyde-fixed tissue sections taken from early and late stages of pregnancy demonstrated that FLIP proteins are present in caspase-8-expressing cells and that expression patterns of FLIP differed according to cell lineage and stage of cell differentiation. The results of this study are consistent with the postulate that FLIP proteins have critical roles in placental cell survival and suggest that FLIP may protect normal and transformed trophoblast cells from cell death.
H Ka; J S Hunt
Publication Detail:
Type:  Journal Article     Date:  2005-09-19
Journal Detail:
Title:  Placenta     Volume:  27     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2006 Jun-Jul
Date Detail:
Created Date:  2006-05-01     Completed Date:  2006-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  626-34     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA.
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MeSH Terms
CASP8 and FADD-Like Apoptosis Regulating Protein
Caspase 8
Caspases / metabolism
Cell Line, Tumor
Choriocarcinoma / metabolism,  pathology
Extraembryonic Membranes / cytology,  metabolism*
Gene Expression Regulation, Developmental
Gestational Age*
Immunoenzyme Techniques
Intracellular Signaling Peptides and Proteins / genetics,  metabolism*
Pregnancy Trimester, First
Protein Isoforms
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Term Birth
Trophoblasts / cytology,  metabolism*
Reg. No./Substance:
0/CASP8 and FADD-Like Apoptosis Regulating Protein; 0/CFLAR protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/Protein Isoforms; 0/RNA, Messenger; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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