| FLICE-inhibitory protein: expression in early and late gestation human placentas. | |
| | |
MedLine Citation:
|
PMID: 16174530 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The apoptosis cascade that plays a central role in normal and pathological processes is strictly controlled, in part by FLIP (Fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein), an inhibitor of caspase-8. Here, we report the expression of long and short isoforms of FLIP mRNAs and proteins in early and late gestation human placentas, term cytotrophoblast cells and two choriocarcinoma cell lines, JEG-3 and Jar. Reverse transcriptase polymerase chain reaction identified mRNAs derived from the FLIP gene in all samples. Analysis by immunoblotting revealed that both long and short forms of FLIP proteins are present in early and late gestation human placentas with increasing levels over gestation and that FLIP proteins are present in normal and transformed trophoblast cells. Immunohistochemical experiments performed on paraformaldehyde-fixed tissue sections taken from early and late stages of pregnancy demonstrated that FLIP proteins are present in caspase-8-expressing cells and that expression patterns of FLIP differed according to cell lineage and stage of cell differentiation. The results of this study are consistent with the postulate that FLIP proteins have critical roles in placental cell survival and suggest that FLIP may protect normal and transformed trophoblast cells from cell death. |
| | |
Authors:
|
H Ka; J S Hunt |
Publication Detail:
|
Type: Journal Article Date: 2005-09-19 |
Journal Detail:
|
Title: Placenta Volume: 27 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2006 Jun-Jul |
Date Detail:
|
Created Date: 2006-05-01 Completed Date: 2006-06-22 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
|
Languages: eng Pagination: 626-34 Citation Subset: IM |
Affiliation:
|
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA. hka@dragon.yonsei.ac.kr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult CASP8 and FADD-Like Apoptosis Regulating Protein Caspase 8 Caspases / metabolism Cell Line, Tumor Choriocarcinoma / metabolism, pathology Extraembryonic Membranes / cytology, metabolism* Female Gene Expression Regulation, Developmental Gestational Age* Humans Immunoenzyme Techniques Intracellular Signaling Peptides and Proteins / genetics, metabolism* Pregnancy Pregnancy Trimester, First Protein Isoforms RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Term Birth Trophoblasts / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/CASP8 and FADD-Like Apoptosis Regulating Protein; 0/CFLAR protein, human; 0/Intracellular Signaling Peptides and Proteins; 0/Protein Isoforms; 0/RNA, Messenger; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Wear reduction of orthopaedic bearing surfaces using polyelectrolyte multilayer nanocoatings.
Next Document: Isolation and Identification of a Multilineage Potential Mesenchymal Cell from Human Placenta.