| FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy. | |
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MedLine Citation:
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PMID: 18677100 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy. Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin. Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle. |
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Authors:
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Lucilla Bongiorno-Borbone; Antonella De Cola; Patrizia Vernole; Livio Finos; Daniela Barcaroli; Richard A Knight; Gerry Melino; Vincenzo De Laurenzi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-21 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 7 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-08 Completed Date: 2008-11-24 Revised Date: 2012-02-24 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 2357-67 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine, University of Rome Tor Vergata, Roma, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aneuploidy Apoptosis Regulatory Proteins / metabolism* Calcium-Binding Proteins / metabolism* Cell Cycle / physiology Cell Cycle Proteins / metabolism* Cells, Cultured Coiled Bodies / metabolism* Cytogenetic Analysis HCT116 Cells HeLa Cells Humans Nuclear Proteins / metabolism* Ploidies* |
| Grant Support | |
ID/Acronym/Agency:
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GGP04110//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/CASP8AP2 protein, human; 0/Calcium-Binding Proteins; 0/Cell Cycle Proteins; 0/NPAT protein, human; 0/Nuclear Proteins; 136882-81-0/p80-coilin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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