Document Detail


FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy.
MedLine Citation:
PMID:  18677100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy. Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin. Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle.
Authors:
Lucilla Bongiorno-Borbone; Antonella De Cola; Patrizia Vernole; Livio Finos; Daniela Barcaroli; Richard A Knight; Gerry Melino; Vincenzo De Laurenzi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-21
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  7     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-08     Completed Date:  2008-11-24     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2357-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Apoptosis Regulatory Proteins / metabolism*
Calcium-Binding Proteins / metabolism*
Cell Cycle / physiology
Cell Cycle Proteins / metabolism*
Cells, Cultured
Coiled Bodies / metabolism*
Cytogenetic Analysis
HCT116 Cells
HeLa Cells
Humans
Nuclear Proteins / metabolism*
Ploidies*
Grant Support
ID/Acronym/Agency:
GGP04110//Telethon; MC_U132670600//Medical Research Council
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/CASP8AP2 protein, human; 0/Calcium-Binding Proteins; 0/Cell Cycle Proteins; 0/NPAT protein, human; 0/Nuclear Proteins; 136882-81-0/p80-coilin

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