Document Detail


FISH shows that Desulfotomaculum spp. are the dominating sulfate-reducing bacteria in a pristine aquifer.
MedLine Citation:
PMID:  15085304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hydrochemistry and the microbial diversity of a pristine aquifer system near Garzweiler, Germany, were characterized. Hydrogeochemical and isotopic data indicate a recent activity of sulfate-reducing bacteria in the Tertiary marine sands. The community structure in the aquifer was studied by fluorescence in situ hybridization (FISH). Up to 7.3 x 10(5) cells/mL were detected by DAPI-staining. Bacteria (identified by the probe EUB338) were dominant, representing 51.9% of the total cell number (DAPI). Another 25.7% of total cell were affiliated with the domain Archaea as identified by the probe ARCH915. Within the domain Bacteria, the beta-Proteobacteria were most abundant (21.0% of total cell counts). Using genus-specific probes for sulfate-reducing bacteria (SRB), 2.5% of the total cells were identified as members of the genus Desulfotomaculum. This reflects the predominant role these microorganisms have been found to play in sulfate-reducing zones of aquifers at other sites. Previously, all SRB cultured from this site were from the spore-forming genera Desulfotomaculum and Desulfosporosinus.
Authors:
J Detmers; H Strauss; U Schulte; A Bergmann; K Knittel; J Kuever
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-10-23
Journal Detail:
Title:  Microbial ecology     Volume:  47     ISSN:  0095-3628     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-09-28     Completed Date:  2004-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  236-42     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Max-Planck-Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Archaea / genetics,  physiology
Desulfotomaculum / genetics,  physiology*
Ecosystem*
Fresh Water / chemistry,  microbiology*
Germany
In Situ Hybridization, Fluorescence
Likelihood Functions
Models, Genetic
Oligonucleotides
Phylogeny*
Sequence Analysis, DNA
Water Microbiology*
Chemical
Reg. No./Substance:
0/Oligonucleotides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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