Document Detail


FISH detection of ribosomal cistrons and assortment-distortion for X and B chromosomes in Dichroplus pratensis (Acrididae).
MedLine Citation:
PMID:  15292606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Assortment-distortion with respect to the X and NOR activity of a rare mitotically stable B chromosome (B(N)), was examined in 16 males of Dichroplus pratensis (Acrididae: Melanoplinae) from Argentine populations. In 1B individuals, the X and B associate preferentially during prophase I reaching a maximum level of association at zygotene. Frequency of X/B association remains relatively high up to diplotene-diakinesis and decreases steeply towards metaphase I. The percent X/B association at each stage is positively influenced by association at the previous stage, and interindividual variability in X/B association decreases as the frequency of association increases. Both chromosomes tended to preferentially orientate toward the same pole at MI (mean ratio of 16 individuals, 1.50:1) which determined an excess of XB and 00 second spermatocytes over X0 and 0B ones (1.39:1). No significant differences occurred between the MI, AI and MII assortment ratios. Fluorescent in situ hybridisation (FISH) confirmed that the B chromosome carries ribosomal genes and helped to establish that, during spermiogenesis, both the B and the normal NOR-bearing chromosome (S8) are clustered near the centriole adjunct region of spermatids. However, FISH failed to reveal the existence of inactive ribosomal cistrons in the X chromosome, as previously suggested, thus providing no support to a simple origin of the B from the X.
Authors:
C J Bidau; M Rosato; D A Martí
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  106     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2004  
Date Detail:
Created Date:  2004-08-04     Completed Date:  2005-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  295-301     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Affiliation:
Laboratorio de Genética Evolutiva, Universidad Nacional de Misiones, Posadas, Argentina. bidau47@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomal Instability / genetics
Chromosomes / genetics*
DNA, Ribosomal / genetics
Female
Genes / genetics*
Grasshoppers / genetics*
In Situ Hybridization, Fluorescence / methods*
Male
Meiosis / genetics
Meiotic Prophase I / genetics
Mitotic Spindle Apparatus / genetics
Nucleolus Organizer Region / genetics
Ribosomes / genetics*
X Chromosome / genetics*
Chemical
Reg. No./Substance:
0/DNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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