Document Detail


FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage.
MedLine Citation:
PMID:  21215265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differentiated trophoblast cell lineages arise from trophoblast stem (TS) cells. To date such a stem cell population has only been established in the mouse. The objective of this investigation was to establish TS cell populations from rat blastocysts. Blastocysts were cultured individually on a feeder layer of rat embryonic fibroblasts (REFs) in fibroblast growth factor-4 (FGF4) and heparin supplemented culture medium. Once cell colonies were established REF feeder layers could be replaced with REF conditioned medium. The blastocyst-derived cell lines, in either proliferative or differentiated states, did not express genes indicative of ICM-derived tissues. In the proliferative state the cells expressed established stem cell-associated markers of TS cells. Cells ceased proliferation and differentiated when FGF4, heparin, and REF conditioned medium were removed. Differentiation was characterized by a decline of stem cell-associated marker gene expression, the appearance of large polyploid cells (trophoblast giant cells), and the expression of trophoblast differentiation-associated genes. Collectively, the data indicate that the rat blastocyst-derived cell lines not only possess many features characteristic of mouse TS cells but also possess some distinct properties. These rat TS cell lines represent valuable new in vitro models for analyses of mechanisms controlling TS cell renewal and differentiation.
Authors:
Kazuo Asanoma; M A Karim Rumi; Lindsey N Kent; Damayanti Chakraborty; Stephen J Renaud; Norio Wake; Dong-Soo Lee; Kaiyu Kubota; Michael J Soares
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-05
Journal Detail:
Title:  Developmental biology     Volume:  351     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-14     Completed Date:  2011-04-04     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  110-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Institute for Reproductive Health and Regenerative Medicine, Department of Pathology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology*
Cell Differentiation*
Cell Lineage*
Female
Fibroblast Growth Factor 4 / physiology*
Male
Mice
Phenotype
Rats
Rats, Sprague-Dawley
Stem Cells / cytology*
Trophoblasts / cytology*
Grant Support
ID/Acronym/Agency:
HD20676/HD/NICHD NIH HHS; HD48861/HD/NICHD NIH HHS; R01 HD020676-22/HD/NICHD NIH HHS; R01 HD020676-23/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fgf4 protein, rat; 0/Fibroblast Growth Factor 4
Comments/Corrections

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