Document Detail


FGF23 is processed by proprotein convertases but not by PHEX.
MedLine Citation:
PMID:  15268897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
X-linked hypophosphatemia (XLH) and autosomal dominant hypophosphatemic rickets (ADHR) are characterized by renal phosphate wasting, rickets, and osteomalacia. ADHR is caused by gain of function mutations in the fibroblast growth factor 23 gene (FGF23). During secretion, FGF23 is processed at the C-terminus between amino acids 179 and 180. The cleavage site is mutated in ADHR, preventing processing of FGF23. Here, we show that FGF23 is likely to be cleaved by subtilisin-like proprotein convertases (SPC) as cleavage can be inhibited by a specific SPC inhibitor in HEK293 cells. SPCs, which are widely expressed, were demonstrated to be also present in HEK293 cells as well as in osteoblasts. XLH is caused by loss of function mutations in the putative endopeptidase PHEX. It was tempting to speculate that FGF23 is a substrate of PHEX, but studies have been inconclusive so far. Here, we used a secreted form of PHEX (secPHEX) and tagged and untagged FGF23 constructs for co-incubation experiments. These experiments provided evidence against cleavage of intact FGF23(25-251) as well as of N-terminal (FGF23(25-179)) and C-terminal (FGF23(180-251)) fragments by the endopeptidase PHEX.
Authors:
Anna Benet-Pagès; Bettina Lorenz-Depiereux; Hans Zischka; Kenneth E White; Michael J Econs; Tim M Strom
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bone     Volume:  35     ISSN:  8756-3282     ISO Abbreviation:  Bone     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-22     Completed Date:  2005-02-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8504048     Medline TA:  Bone     Country:  United States    
Other Details:
Languages:  eng     Pagination:  455-62     Citation Subset:  IM    
Affiliation:
Institute of Human Genetics, GSF National Research Center, 85764 München-Neuherberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Line
DNA Primers
Fibroblast Growth Factors / metabolism*
Humans
PHEX Phosphate Regulating Neutral Endopeptidase
Proprotein Convertases / metabolism*
Protein Processing, Post-Translational*
Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
K24-AR02095/AR/NIAMS NIH HHS; R01AR42228/AR/NIAMS NIH HHS; R01DK063934/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Proteins; 0/fibroblast growth factor 23; 62031-54-3/Fibroblast Growth Factors; EC 3.4.-/Proprotein Convertases; EC 3.4.24.-/PHEX Phosphate Regulating Neutral Endopeptidase; EC 3.4.24.-/PHEX protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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