| FGF2-labeled semiconductor nanocrystals as luminescent biolabels for imaging neuroblastoma cells. | |
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MedLine Citation:
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PMID: 21361128 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In this paper, we report the synthesis of FGF-2 peptide labeled luminescent nanocrystals as optical probes for targeted imaging of live neuroblastoma cells. Confocal laser scanning microscopy technique was used to confirm the receptor-mediated uptake of the bioconjugates into the live neuroblastoma cells and study the dynamic profile of the bioconjugates within a single cell. It was found that the bioconjugates were self-assembled near the nuclear periphery after they entered the cellular cytoplasm. In addition to confocal imaging, the cytotoxicity of bioconjugates was investigated using colorimetric cell viability (MTS) assay. The MTS study reveals that the bioconjugate formulation has very low toxicity. These results demonstrate that the luminescent nanocrystal bioconjugates can serve as biocompatible targeted probes for multiplex cell labeling and molecule tracking. |
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Authors:
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Gaixia Xu; Ken-Tye Yong; Indrajit Roy; Atcha Kopwitthaya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical nanotechnology Volume: 6 ISSN: 1550-7033 ISO Abbreviation: J Biomed Nanotechnol Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-03-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101230869 Medline TA: J Biomed Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 641-7 Citation Subset: IM |
Affiliation:
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Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, Institute of Optoelectronics, Shenzhen University, Shenzhen 518060, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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