| FGF signalling in the early specification of mesoderm in Xenopus. | |
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MedLine Citation:
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PMID: 8223274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined the role of FGF signalling in the development of muscle and notochord and in the expression of early mesodermal markers in Xenopus embryos. Disruption of the FGF signalling pathway by expression of a dominant negative construct of the FGF receptor (XFD) generally results in gastrulation defects that are later evident in the formation of the trunk and tail, though head structures are formed nearly normally. These defects are reflected in the loss of notochord and muscle. Even in embryos that show mild defects and gastrulate properly, muscle formation is impaired, suggesting that morphogenesis and tissue differentiation each depend on FGF. The XFD protein inhibits the expression of the immediate early gene brachyury throughout the marginal zone, including the dorsal side; it does not, however, inhibit the dorsal lip marker goosecoid, which is expressed in the first involuting mesoderm at the dorsal side that will underlie the head. The XFD protein also inhibits Xpo expression, an immediate early marker of ventral and lateral mesoderm. These results suggest that FGF is involved in the earliest events of most mesoderm induction that occur before gastrulation and that the early dorsal mesoderm is already composed of two cell populations that differ in their requirements for FGF. |
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Authors:
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E Amaya; P A Stein; T J Musci; M W Kirschner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 118 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-12-09 Completed Date: 1993-12-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 477-87 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, University of California, San Francisco 94143. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Collagen / genetics Embryonic Induction / physiology* Fibroblast Growth Factors / physiology* Gastrula / physiology Gene Expression / physiology Immunoblotting Immunohistochemistry In Situ Hybridization Mesoderm / physiology* Morphogenesis / physiology Muscles / embryology Notochord / cytology Receptors, Fibroblast Growth Factor / genetics, physiology Signal Transduction / physiology* Xenopus laevis / embryology |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Fibroblast Growth Factor; 62031-54-3/Fibroblast Growth Factors; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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