Document Detail


FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor.
MedLine Citation:
PMID:  9707175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The spindle-shaped cell line TTB was recently isolated from highly vascularized skin lesions of BKV/HIV-1 tat transgenic mice and shown to possess an autocrine loop for hepatocyte growth factor (HGF). We show that fibroblast growth factor-2 (FGF-2) stimulates TTB cell migration and promotes polarization of uPAR at the leading edge of migrating cells. FGF-stimulated TTB cells presented the typical migratory phenotype, with a triangular cell shape and concomitant breakdown of actin stress fibers and smooth muscle-specific actin isoform. FGF-2-stimulated migration was blocked by antibodies against urokinase-type plasminogen activator (uPA) or uPA receptor (uPAR) and by neutralizing anti-HGF antibodies. The latter also inhibited uPAR relocalization at the cell surface of FGF-2-treated TTB cells. This points to a crosstalk between FGF-2 and HGF that might mediate TTB cell migration by modulating the localization of cell surface uPAR.
Authors:
U Cavallaro; Z Wu; A Di Palo; R Montesano; M S Pepper; J A Maier; M R Soria
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  12     ISSN:  0892-6638     ISO Abbreviation:  FASEB J.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-02     Completed Date:  1998-09-02     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1027-34     Citation Subset:  IM; X    
Affiliation:
Department of Biological and Technological Research, San Raffaele Scientific Institute, Milano, Italy. cavallu@dibit.hsr.it
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Movement*
Cytoskeleton
Fibroblast Growth Factor 2 / pharmacology*
Hepatocyte Growth Factor / metabolism*,  secretion
Receptors, Cell Surface / metabolism*
Receptors, Urokinase Plasminogen Activator
Sarcoma, Kaposi
Urokinase-Type Plasminogen Activator / metabolism
Chemical
Reg. No./Substance:
0/Plaur protein, mouse; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; 103107-01-3/Fibroblast Growth Factor 2; 67256-21-7/Hepatocyte Growth Factor; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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