Document Detail


FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for In Vitro Contamination.
MedLine Citation:
PMID:  22505908     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective. To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation. Methods. Twenty-five human single-rooted teeth were used. The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (n = 5), according to the preparation methods employed-control group: no solutions applied; group 1: cement removal and irrigation with 5.25% NaOCl  + 17 % EDTA for 4 minutes each; group 2: 17%  EDTA + 2.5% NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17%  EDTA + 5.25%  NaOCl + phosphate buffer solution + distilled water (10 minutes ultrasonic); group 4: 17%  EDTA + 5.25% NaOCl (3 minutes ultrasonic bath). Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification. Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test. Results. In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4. Conclusion. The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.
Authors:
Vitor Cesar Nakamura; Simony Hidee Hamoy Kataoka; Giulio Gavini; Patrícia Helena Ferrari; Silvana Cai
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Publication Detail:
Type:  Journal Article     Date:  2012-03-11
Journal Detail:
Title:  International journal of dentistry     Volume:  2012     ISSN:  1687-8736     ISO Abbreviation:  Int J Dent     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524183     Medline TA:  Int J Dent     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  748471     Citation Subset:  -    
Affiliation:
Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
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