| FCS-based sensing for the detection of ochratoxin and neomycin in food. | |
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MedLine Citation:
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PMID: 20001903 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively. |
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Authors:
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Antonio Varriale; Maria Staiano; Luisa Iozzino; Lorella Severino; Aniello Anastasio; Maria Luisa Cortesi; Sabato D'Auria |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein and peptide letters Volume: 16 ISSN: 1875-5305 ISO Abbreviation: Protein Pept. Lett. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-12-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9441434 Medline TA: Protein Pept Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1425-8 Citation Subset: IM |
Affiliation:
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Laboratory for Molecular Sensing, IBP-CNR, Naples, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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