Document Detail


FCS-based sensing for the detection of ochratoxin and neomycin in food.
MedLine Citation:
PMID:  20001903     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.
Authors:
Antonio Varriale; Maria Staiano; Luisa Iozzino; Lorella Severino; Aniello Anastasio; Maria Luisa Cortesi; Sabato D'Auria
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein and peptide letters     Volume:  16     ISSN:  1875-5305     ISO Abbreviation:  Protein Pept. Lett.     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1425-8     Citation Subset:  IM    
Affiliation:
Laboratory for Molecular Sensing, IBP-CNR, Naples, Italy.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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