Document Detail


FACIT collagen (1alpha-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis.
MedLine Citation:
PMID:  18045687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Based on previous cloning and sequencing study, real-time PCR and in situ hybridization assays of the inflamed body wall of LPS-injected Ciona intestinalis showed the enhanced gene expression of a collagen with FACIT structural features (Ci-type IX-Col 1alpha-chain). By using specific antibodies raised against an opportunely chosen Ci-type IX-Col synthetic peptide, the fibroblast property of hemocytes challenged in vitro with LPS (at 4h) was displayed by flow cytometry, while immunocytochemistry identified hemocytes with large granules (morula cells) as collagen-producing cells. Hemocyte lysate supernatant analyzed in immunoblotting contained a 60 kDa band identifiable as 1alpha-chain-Ci-type IX-Col. Observations of body wall sections (immunohistochemistry method) supported the role of hemocytes and showed that epidermis expressed Ci-type IX-Col 1alpha-chain in the time course of the inflammatory reaction (within 24h). Transcript and protein were mainly found in the epidermis that outlined the proximal side of the tunic matrix (at 24h after LPS injection), in cells associated with the epidermis at 4 and 192 h. In conclusion, the C. intestinalis inflammatory response to LPS challenge appeared to be composed of a complex reaction set, and for the first time we showed in ascidians a granulation tissue with FACIT-collagen production that could participate in inflammation and wound healing. Like in vertebrates, C. intestinalis acute inflammatory reactions result in a regulated pattern of tissue repair with collagen expression during remodelling. Ci-type IX-Col could be involved in a network of non-fibril-forming collagens that participates in the organization of extracellular matrix and defense responses.
Authors:
Aiti Vizzini; Margherita Pergolizzi; Mirella Vazzana; Giuseppina Salerno; Caterina Di Sano; Pasquale Macaluso; Vincenzo Arizza; Daniela Parrinello; Matteo Cammarata; Nicolò Parrinello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-09
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  32     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-03     Completed Date:  2008-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  682-92     Citation Subset:  IM    
Affiliation:
Department of Animal Biology, University of Palermo, Palermo, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ciona intestinalis*
Collagen Type IX / biosynthesis*,  immunology*,  metabolism
Epidermis / cytology,  immunology*,  metabolism
Escherichia coli
Extracellular Matrix / metabolism
Gene Expression Profiling
Hemocytes / cytology,  immunology*,  metabolism
Immunohistochemistry
In Situ Hybridization
Inflammation
Lipopolysaccharides / pharmacology
Paracrine Communication
Procollagen / biosynthesis,  immunology,  metabolism
Protein Processing, Post-Translational / drug effects
Chemical
Reg. No./Substance:
0/Collagen Type IX; 0/Lipopolysaccharides; 0/Procollagen

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