Document Detail

F reticulocytes assay: a method to evaluate fetal hemoglobin production.
MedLine Citation:
PMID:  9859926     Owner:  NLM     Status:  MEDLINE    
Production of fetal hemoglobin (Hb F) involves molecular as well as cellular aspects as, among red blood cells, it is restricted to a specific population referred as the F cells. Thus understanding the mechanisms involved in persistence or re-emergence of Hb F production in various inherited or acquired conditions requires the measurement of both Hb F and F cells. In addition, in disorders with a hemolytic component, including sickle cell disease (SCD), because of a probable preferential survival of F cells as compared to non-F cells, the true parameter of F cell production is the F reticulocyte count. The F cells/F reticulocytes ratio then selectively reflects this preferential survival. Here we describe an original immunofluorescence microscopy assay that permits the simultaneous measurement of F cells and F reticulocytes. For this assay to be widely usable, we chose to use commercially available monoclonal antibodies.
M Maier-Redelsperger; J Elion; R Girot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hemoglobin     Volume:  22     ISSN:  0363-0269     ISO Abbreviation:  Hemoglobin     Publication Date:    1998 Sep-Nov
Date Detail:
Created Date:  1999-02-24     Completed Date:  1999-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705865     Medline TA:  Hemoglobin     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  419-25     Citation Subset:  IM    
Service d'Hématologie Biologique, Hôpital Tenon, Paris, France.
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MeSH Terms
Anemia, Sickle Cell / blood
Antibodies, Monoclonal
Child, Preschool
Erythrocyte Count
Fetal Hemoglobin / analysis*,  biosynthesis,  immunology
Fluorescent Antibody Technique*
Infant, Newborn
Reticulocytes / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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