| An F-derived conjugative cosmid: analysis of tra polypeptides in cosmid-infected cells. | |
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MedLine Citation:
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PMID: 2944151 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The genes involved in the conjugational transfer of F plasmid DNA are organized into three closely linked operons spanning an overall length of approximately 33 kilobase pairs of F. The entire transfer (tra) region comprising all three operons has been cloned into the cosmid vector pHC79 by in vitro recombination and packaging techniques. The transfer-proficient chimeric cosmid pRS2405 was packaged into lambda capsids, and uv-irradiated E. coli cells were infected with these DNA-filled particles. A number of polypeptides programmed by the infecting DNA were identified as tra-specified products; a traJ90 mutation on pRS2405 resulted in the significant reduction of synthesis of all detectable pRS2405-specified tra polypeptides, with the exception of TraTp. |
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Authors:
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A Ray; K C Cheah; R Skurray |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plasmid Volume: 16 ISSN: 0147-619X ISO Abbreviation: Plasmid Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1986-10-22 Completed Date: 1986-10-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7802221 Medline TA: Plasmid Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 90-100 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics Bacteriophage lambda / genetics Cloning, Molecular Conjugation, Genetic* Cosmids* DNA Replication F Factor* Gene Expression Regulation Genes, Bacterial Genetic Vectors Morphogenesis Promoter Regions, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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