Document Detail


Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis.
MedLine Citation:
PMID:  16458879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. Here, we studied the effects caused by loss of eya1 and six1 function on pituitary development in zebrafish. eya1 and six1 are co-expressed in all adenohypophyseal cells. Nevertheless, eya1 (aal, dog) mutants show lineage-specific defects, defining corticotropes, melanotropes, and gonadotropes as an Eya1-dependent lineage, which is complementary to the Pit1 lineage. Furthermore, eya1 is required for maintenance of pit1 expression, leading to subsequent loss of cognate hormone gene expression in thyrotropes and somatotropes of mutant embryos, whereas prolactin expression in lactotropes persists. In contrast to other organs, adenohypophyseal cells of eya1 mutants do not become apoptotic, and the adenohypophysis remains at rather normal size. Also, cells do not trans-differentiate, as in the case of pit1 mutants, but display morphological features characteristic for nonsecretory cells. Some of the adenohypophyseal defects of eya1 mutants are moderately enhanced in combination with antisense-mediated loss of Six1 function, which per se does not affect pituitary cell differentiation. In conclusion, this is the first report of an essential role of Eya1 during pituitary development in vertebrates. Eya1 is required for lineage-specific differentiation of adenohypophyseal cells, but not for their survival, thereby uncoupling the differentiation-promoting and anti-apoptotic effects of Eya proteins seen in other tissues.
Authors:
Gabriela Nica; Wiebke Herzog; Carmen Sonntag; Matthias Nowak; Heinz Schwarz; Agustin G Zapata; Matthias Hammerschmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-03
Journal Detail:
Title:  Developmental biology     Volume:  292     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-05-11     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-204     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Apoptosis / genetics,  physiology
Cell Differentiation / genetics,  physiology*
Cell Lineage / genetics,  physiology*
Cell Survival / genetics,  physiology
Gene Expression Regulation, Developmental / physiology
Growth Hormone / biosynthesis,  genetics
Homeodomain Proteins / biosynthesis,  genetics,  physiology
Intracellular Signaling Peptides and Proteins / genetics,  physiology*
LIM-Homeodomain Proteins
Molecular Sequence Data
Mutation
Nerve Tissue Proteins / biosynthesis,  genetics
Nuclear Proteins / genetics,  physiology*
Pituitary Gland, Anterior / cytology*,  embryology*
Pro-Opiomelanocortin / biosynthesis,  genetics
Protein Tyrosine Phosphatases / genetics,  physiology*
Thyrotropin / biosynthesis,  genetics
Transcription Factor Pit-1 / biosynthesis,  genetics
Transcription Factors / biosynthesis,  genetics
Zebrafish / embryology*,  genetics
Zebrafish Proteins / biosynthesis,  genetics,  physiology*
Grant Support
ID/Acronym/Agency:
R01 GM063904/GM/NIGMS NIH HHS; R01 GM063904-01/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/LIM-Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Six1 protein, zebrafish; 0/Transcription Factor Pit-1; 0/Transcription Factors; 0/Zebrafish Proteins; 0/homeobox protein PITX3; 0/lhx3 protein, zebrafish; 66796-54-1/Pro-Opiomelanocortin; 9002-71-5/Thyrotropin; 9002-72-6/Growth Hormone; EC 3.1.3.48/Protein Tyrosine Phosphatases; EC 3.1.3.48/eya1 protein, zebrafish

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