Document Detail

Extreme stability of Tyr-MIF-1 in CSF.
MedLine Citation:
PMID:  7970149     Owner:  NLM     Status:  MEDLINE    
Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2), an endogenous brain peptide, was incubated at 37 degrees C in cerebrospinal fluid (CSF) from rats and the amount remaining in intact from determined by high performance liquid chromatography (HPLC). By 3 min, a time at which Tyr-MIF-1 had been considerably degraded by rat plasma, essentially no degradation of the tetrapeptide had occurred in CSF. Tyr-MIF-1 persisted in mainly intact form for the 8 days tested, with a calculated half-time degradation of 11.4 days. The simultaneous addition to CSF of equal amounts of Tyr-MIF-1 tritiated on the Tyr and Tyr-MIF-1 tritiated on the Pro facilitated determination of whether Tyr-MIF-1 serves as a precursor of MIF-1 (Pro-Leu-Gly-NH2) in CSF. This possibility was excluded by the findings that the primary metabolite was Tyr-Pro, with minimal and equal amounts of the free amino acids Pro and Tyr being formed, but no MIF-1. The results show the extreme stability of Tyr-MIF-1 in CSF and are consistent with a role for this peptide in the CNS.
A J Kastin; W A Banks; K Hahn; J E Zadina
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  174     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-12-07     Completed Date:  1994-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  26-8     Citation Subset:  IM    
VA Medical Center, New Orleans, LA.
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MeSH Terms
Amino Acid Sequence
Chromatography, High Pressure Liquid
Iodine Isotopes
MSH Release-Inhibiting Hormone / analogs & derivatives*,  cerebrospinal fluid
Molecular Sequence Data
Rats, Sprague-Dawley
Reg. No./Substance:
0/Iodine Isotopes; 77133-61-0/tyrosyl-prolyl-leucyl-glycinamide; 9083-38-9/MSH Release-Inhibiting Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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