Document Detail

Extraglomerular origin of the mesangial cell after injury. A new role of the juxtaglomerular apparatus.
MedLine Citation:
PMID:  9259576     Owner:  NLM     Status:  MEDLINE    
We investigated the origin of the glomerular mesangial cell, a smooth muscle-like cell that provides structural support in the glomerulus. Injection of anti-Thy 1 antibody that binds the Thy 1 antigen on rat mesangial cells eliminated (> 95%) the mesangial population at 20-28 h, while Thy 1-positive cells in the juxtaglomerular apparatus (JGA) were sequestered from the circulation and survived. Single pulse labeling with [3H]thymidine at 36 h labeled Thy 1-positive cells in the JGA and hilus. Serial biopsies demonstrated the progressive migration (5-15 micron/d) and proliferation of these mesangial reserve cells until the entire glomerulus was repopulated. The regenerating mesangial population expressed contractile and migratory proteins preferentially at the leading edge of the migratory front. Single as well as multiple pulse labeling with [3H]thymidine confirmed that the entire mesangial cell repopulation originated from only a few mesangial reserve cells. These reserve cells resided in the extraglomerular mesangium in the JGA and were not renin-secreting cells, macrophages, smooth muscle cells, or endothelial cells. These studies document mesangial cell migration in the anti-Thy 1 model of mesangial proliferative glomerulonephritis and provide evidence for a new role for the juxtaglomerular apparatus in the maintenance of the mesangial cell population.
C Hugo; S J Shankland; D F Bowen-Pope; W G Couser; R J Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  100     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-09     Completed Date:  1997-10-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  786-94     Citation Subset:  AIM; IM    
Division of Nephrology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
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MeSH Terms
Antigens, Surface / metabolism
Cell Movement
Disease Models, Animal
Dose-Response Relationship, Drug
Glomerular Mesangium / metabolism,  pathology*
Glomerulonephritis / pathology*
Isoantibodies / administration & dosage
Juxtaglomerular Apparatus / metabolism,  pathology*
Kidney Glomerulus / metabolism,  pathology*
Rats, Wistar
Time Factors
Grant Support
Reg. No./Substance:
0/Antigens, Surface; 0/Isoantibodies; 0/anti-Thy antibody

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