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Extracytoplasmic function σ factors of the widely distributed group ECF41 contain a fused regulatory domain.
MedLine Citation:
PMID:  22950025     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Bacteria need signal transducing systems to respond to environmental changes. Next to one- and two-component systems, alternative σ factors of the extra-cytoplasmic function (ECF) protein family represent the third fundamental mechanism of bacterial signal transduction. A comprehensive classification of these proteins identified more than 40 phylogenetically distinct groups, most of which are not experimentally investigated. Here, we present the characterization of such a group with unique features, termed ECF41. Among analyzed bacterial genomes, ECF41 σ factors are widely distributed with about 400 proteins from 10 different phyla. They lack obvious anti-σ factors that typically control activity of other ECF σ factors, but their structural genes are often predicted to be cotranscribed with carboxymuconolactone decarboxylases, oxidoreductases, or epimerases based on genomic context conservation. We demonstrate for Bacillus licheniformis and Rhodobacter sphaeroides that the corresponding genes are preceded by a highly conserved promoter motif and are the only detectable targets of ECF41-dependent gene regulation. In contrast to other ECF σ factors, proteins of group ECF41 contain a large C-terminal extension, which is crucial for σ factor activity. Our data demonstrate that ECF41 σ factors are regulated by a novel mechanism based on the presence of a fused regulatory domain.
Authors:
Tina Wecke; Petra Halang; Anna Staroń; Yann S Dufour; Timothy J Donohue; Thorsten Mascher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  MicrobiologyOpen     Volume:  1     ISSN:  2045-8827     ISO Abbreviation:  Microbiologyopen     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-09-05     Completed Date:  2012-09-11     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101588314     Medline TA:  Microbiologyopen     Country:  England    
Other Details:
Languages:  eng     Pagination:  194-213     Citation Subset:  -    
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