Document Detail


Extracts of proliferating and non-proliferating human cells display different base excision pathways and repair fidelity.
MedLine Citation:
PMID:  19442590     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capacity for single- and two-nucleotide insertion BER activity. However, patch size longer than two nucleotides was only detected in extracts from proliferating cells. Relative to extracts from proliferating cells, extracts from non-proliferating cells had approximately two-fold higher concentration of POLbeta, which contributed to most of two-nucleotide insertion BER. In contrast, two-nucleotide insertion in extracts from proliferating cells was not dependent on POLbeta. BER fidelity was two- to three-fold lower in extracts from the non-proliferating compared with extracts of proliferating cells. Furthermore, although one-nucleotide deletion was the predominant type of repair error in both extracts, the pattern of repair errors was somewhat different. These results establish two-nucleotide patch BER as a distinct POLbeta-dependent mechanism in non-proliferating cells and demonstrate that BER fidelity is lower in extracts from non-proliferating as compared with proliferating cells.
Authors:
Mansour Akbari; Javier Peña-Diaz; Sonja Andersen; Nina-Beate Liabakk; Marit Otterlei; Hans Einar Krokan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-12
Journal Detail:
Title:  DNA repair     Volume:  8     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  834-43     Citation Subset:  IM    
Affiliation:
Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, NTNU, N-7489 Trondheim, Norway. mansour.akbari@ntnu.no
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites / genetics
Blotting, Western
Cell Extracts / chemistry
Cell Line
Cell Proliferation*
Cells, Cultured
DNA Polymerase beta / genetics,  metabolism
DNA Repair / physiology*
Humans
Keratinocytes / cytology,  metabolism
Lymphocytes / chemistry,  cytology,  metabolism*
Mutation
Oligonucleotides / genetics,  metabolism
Signal Transduction / physiology*
Substrate Specificity
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Oligonucleotides; EC 2.7.7.-/DNA Polymerase beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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