Document Detail

Extracts of Sarcoptes scabiei De Geer downmodulate secretion of IL-8 by skin keratinocytes and fibroblasts and of GM-CSF by fibroblasts in the presence of proinflammatory cytokines.
MedLine Citation:
PMID:  19645287     Owner:  NLM     Status:  MEDLINE    
Previous in vitro studies showed that molecules in an extract of the mite Sarcoptes scabiei variety canis De Geer could modulate the secretion of cytokines from cultured normal human epidermal keratinocytes and dermal fibroblasts in the absence of proinflammatory cytokines in the cell culture media. The purpose of this study was to investigate whether scabies extract could also modulate cytokine and chemokine secretion from epidermal keratinocytes and dermal fibroblasts in the presence of proinflammatory cytokines that are likely present in the scabietic lesion in vivo. In particular, could the downmodulating properties of this ectoparasitic mite on skin cells be maintained in the presence of proinflammatory cytokines? We found that even in the presence of the proinflammatory cytokines interleukin (IL)-1alpha, IL-beta, and a mixture of tumor necrosis factor (TNF)alpha + IL-17, scabies extract still downregulated the levels of IL-8 secretion from keratinocytes and fibroblasts and of granulocyte/macrophage-colony stimulating factor (GM-CSF) secretion from fibroblasts that were induced by stimulation of the cells with proinflammatory cytokines alone. This study also showed that scabies molecules induced secretions of growth-related oncogene alpha (GROalpha), transforming growth factor alpha (TGFalpha), and cutaneous T-cell attracting chemokine (CTACK) from keratinocytes and IL-6 and granulocyte-colony stimulating factor (G-CSF) from fibroblasts. These findings, coupled with the previous findings that molecules in scabies extract could downregulate expression of intracellular adhesion molecule-1 (ICAM-1) and E-selectin by normal dermal microvascular endothelial cells and secretion of IL-1alpha from keratinocytes, suggest that multiple factors from scabies mites play a role in the characteristic delayed inflammatory response to a primary infestation with S. scabiei. These are adaptations that favor invasion of the host by the parasite.
Jeremi S Mullins; Larry G Arlian; Marjorie S Morgan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of medical entomology     Volume:  46     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-09-14     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  845-51     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Chemokine CCL27 / secretion
Cytokines / pharmacology*
Fibroblasts / drug effects,  immunology
Granulocyte-Macrophage Colony-Stimulating Factor / secretion*
Interleukin-8 / secretion*
Keratinocytes / drug effects*,  immunology
Sarcoptes scabiei / chemistry*
Tissue Extracts / pharmacology*
Grant Support
Reg. No./Substance:
0/Chemokine CCL27; 0/Cytokines; 0/Interleukin-8; 0/Tissue Extracts; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

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