Document Detail

Extracts of Cynomorium songaricum protect human neuroblastoma cells from beta-amyloid25-35 and superoxide anion induced injury.
MedLine Citation:
PMID:  19827306     Owner:  NLM     Status:  MEDLINE    
In Traditional Chinese Medicine a number of herbs are used to alleviate age-related diseases including memory impairment and dementia, among them stems of Cynomorium songaricum, Cynomoriaceae. In this study, we evaluated the protective effect of different extracts of aerial parts of C. songaricum on amyloid-beta peptide (Abeta) and hypoxanthine/xanthine oxidase induced cell death in SK-N-SH neuroblastoma cells. Abeta (20 microM) as well as superoxide anions generated by the hypoxanthine/xanthine oxidase system both reduced cell viability to about 60%. The methanolic extract of C. songaricum attenuated Abeta induced cell death at concentrations of 100 and 10 microg/ml, an even stronger effect was observed for the ethyl acetate fraction obtained from the crude methanolic extract. On the other hand, the dichloromethane as well as water fractions showed no protective effects. In order to further analyze the protective mode of action, the ability of extracts to protect against superoxide anions induced cell death was also evaluated. In this system, cell viability could again be restored by methanol and ethyl acetate extracts, the latter showingsignificant protective effects even at concentrations as low as 0.1 microg/ml.
Y Lu; Q Wang; M F Melzig; K Jenett-Siems
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Die Pharmazie     Volume:  64     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-10-15     Completed Date:  2009-11-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  609-12     Citation Subset:  IM    
Beijing University of Chinese Medicine, Beijing, P.R. China.
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MeSH Terms
Amyloid beta-Protein / antagonists & inhibitors*,  toxicity*
Cell Line, Tumor
Cell Survival / drug effects
Cynomorium / chemistry*
Neuroprotective Agents*
Oxidants / toxicity*
Peptide Fragments / antagonists & inhibitors*,  toxicity*
Plant Extracts / pharmacology
Superoxides / toxicity*
Xanthine Oxidase / toxicity
Reg. No./Substance:
0/Acetates; 0/Amyloid beta-Protein; 0/Neuroprotective Agents; 0/Oxidants; 0/Peptide Fragments; 0/Plant Extracts; 0/Solvents; 0/amyloid beta-protein (25-35); 11062-77-4/Superoxides; 141-78-6/ethyl acetate; 67-56-1/Methanol; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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