| Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5'-hydroxypiroxicam in human plasma and urine. | |
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MedLine Citation:
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PMID: 8925068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40 degrees C on a C18 Spherisorb column with acetonitrile--0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5'-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 micrograms/ml using a 100-microliters loop. |
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Authors:
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A Avgerinos; S Axarlis; J Dragatsis; T Karidas; S Malamataris |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 673 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-11-13 Completed Date: 1996-11-13 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 142-6 Citation Subset: IM |
Affiliation:
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Military Pharmaceutical Laboratories, Athens, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents, Non-Steroidal
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analysis*,
blood,
urine Chromatography, High Pressure Liquid* / methods Humans Naproxen / analysis Piroxicam / analogs & derivatives*, analysis*, blood, pharmacokinetics, urine Reproducibility of Results Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 22204-53-1/Naproxen; 36322-90-4/Piroxicam; 77459-78-0/5'-hydroxypiroxicam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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