Document Detail


Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5'-hydroxypiroxicam in human plasma and urine.
MedLine Citation:
PMID:  8925068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40 degrees C on a C18 Spherisorb column with acetonitrile--0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5'-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 micrograms/ml using a 100-microliters loop.
Authors:
A Avgerinos; S Axarlis; J Dragatsis; T Karidas; S Malamataris
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  673     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-11-13     Completed Date:  1996-11-13     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  142-6     Citation Subset:  IM    
Affiliation:
Military Pharmaceutical Laboratories, Athens, Greece.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / analysis*,  blood,  urine
Chromatography, High Pressure Liquid* / methods
Humans
Naproxen / analysis
Piroxicam / analogs & derivatives*,  analysis*,  blood,  pharmacokinetics,  urine
Reproducibility of Results
Spectrophotometry
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 22204-53-1/Naproxen; 36322-90-4/Piroxicam; 77459-78-0/5'-hydroxypiroxicam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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