| Extraction optimization and in vivo antioxidant activities of exopolysaccharide by Morchella esculenta SO-01. | |
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MedLine Citation:
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PMID: 20153962 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Response surface methodology was used to optimize extraction parameters for Morchella esculenta SO-01 exopolysaccharide (EPS) produced during submerged culture. The optimum conditions for EPS extraction were predicted to be, concentration at 84.07 degrees C, precipitation for 22.19 h and pH 8.44, and EPS production was estimated at 5.45 g/L. The actual yield of EPS under these conditions was 5.32 g/L. The antioxidant capacity of the EPS was measured in vivo after filling the stomach with different doses of EPS and results showed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of blood, spleen, liver, heart, and kidney were increased by 125%, 46.11%, 23.33%, 12.19%, 41.29%, and 63.24%, 63.12%, 166.54%, 98.01%, 57.68%, respectively, and that malonaldehyde (MDA) of blood, spleen, liver, heart, and kidney were decreased by 21.80%, 67.84%, 28.48%, 56.15%, 41.62%. The results provide a reference for large-scale extraction of EPS by M. esculenta SO-01 in industrial fermentation. |
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Authors:
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Fanyun Meng; Bo Zhou; Rongshan Lin; Le Jia; Xiaonan Liu; Peng Deng; Keming Fan; Guoyi Wang; Li Wang; Jianjun Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-13 |
Journal Detail:
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Title: Bioresource technology Volume: 101 ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-06-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: England |
Other Details:
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Languages: eng Pagination: 4564-9 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Agaricales
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chemistry* Analysis of Variance Animals Antioxidants / isolation & purification*, pharmacology* Biotechnology / methods* Glutathione Peroxidase / metabolism Malondialdehyde / metabolism Mice Organ Specificity / drug effects Polysaccharides / isolation & purification*, pharmacology* Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Polysaccharides; 542-78-9/Malondialdehyde; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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