Document Detail


Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy.
MedLine Citation:
PMID:  6429337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells of the bacterium Acholeplasma laidlawii were rapidly frozen against a copper block cooled by liquid helium. The frozen cells were transferred to liquid nitrogen and subsequently to acetone or methanol at 183 K. After 24 h the cells were infiltrated at 203 K with a non-polar methacrylate resin of the same type as Lowicryl HM20. The resin was cured at the same temperature. Acetone extracted approximately 5% of the lipid content of the cells, methanol 15-45% and the resin only negligible amounts. Similar results were obtained with A. laidlawii-ghosts. The cells appeared well preserved when examined in the electron microscope.
Authors:
C Weibull; W Villiger; E Carlemalm
Related Documents :
19494637 - Cryopreservation for corneal storage.
21258487 - Optical injection of mammalian cells using a microfluidic platform.
3816167 - A voice-entry data management system: application in monoclonal antibody research.
11934307 - Modeling of cryopreservation of engineered tissues with one-dimensional geometry.
17591847 - Paradoxical growth effect of caspofungin observed on biofilms and planktonic cells of f...
19933277 - Beta-catenin/t-cell factor signaling is activated during lung injury and promotes the s...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  134     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-08-15     Completed Date:  1984-08-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acholeplasma laidlawii / ultrastructure*
Freezing
Glutaral
Indicators and Reagents
Lipids / isolation & purification*
Microscopy, Electron / methods
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Lipids; 111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Liquid propane jet-freezing, freeze-drying and rotary replication of the cytoskeleton and plasma mem...
Next Document:  The phylogeny of the hominoid primates, as indicated by DNA-DNA hybridization.