Document Detail

Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy.
MedLine Citation:
PMID:  6429337     Owner:  NLM     Status:  MEDLINE    
Cells of the bacterium Acholeplasma laidlawii were rapidly frozen against a copper block cooled by liquid helium. The frozen cells were transferred to liquid nitrogen and subsequently to acetone or methanol at 183 K. After 24 h the cells were infiltrated at 203 K with a non-polar methacrylate resin of the same type as Lowicryl HM20. The resin was cured at the same temperature. Acetone extracted approximately 5% of the lipid content of the cells, methanol 15-45% and the resin only negligible amounts. Similar results were obtained with A. laidlawii-ghosts. The cells appeared well preserved when examined in the electron microscope.
C Weibull; W Villiger; E Carlemalm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  134     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-08-15     Completed Date:  1984-08-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-6     Citation Subset:  IM    
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MeSH Terms
Acholeplasma laidlawii / ultrastructure*
Indicators and Reagents
Lipids / isolation & purification*
Microscopy, Electron / methods
Reg. No./Substance:
0/Indicators and Reagents; 0/Lipids; 111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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