| Extraction of label during processing of brain tissue for electron microscopic autoradiographic investigation of glycoprotein metabolism. | |
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MedLine Citation:
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PMID: 6757197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rats were injected intracerebrally with (3H) fucose and survived for 30 min and 24 h. Following perfusion fixation with aldehydes, Vibratome sections of the brains were processed for embedding in resin and the radioactivity in the various solutions measured by scintillation counting. The decline of radioactivity was monitored in up to 18 successive buffer washes. Whereas 85% of the initial radioactivity could be eluted after 30 min, only 7% were extractable after 24 h survival time. Addition of unlabelled L-fucose to the fixative and buffers caused a small insignificant increase of the total radioactivity extracted. Only small amounts of radioactivity could be removed by treatment with trichloroacetic acid after extensive rinsing in buffer. It is concluded that thorough rinsing in buffer (which is more important with short-time experiments) is effective in removing the acid-soluble radioactivity. An additional safe-guard lies in dehydration by ethanol. The ethanol series also removes acid-soluble radioactivity in addition to small amounts of what is probably higher molecular weight material. |
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Authors:
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C Pilgrim; I Saalmüller; I Reisert |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 74 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1982 |
Date Detail:
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Created Date: 1983-02-14 Completed Date: 1983-02-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 271-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Brain / metabolism*, ultrastructure Buffers Fucose / diagnostic use* Glycoproteins / metabolism* Histological Techniques* Male Microscopy, Electron Rats Rats, Inbred Strains Scintillation Counting / methods Time Factors Tritium / diagnostic use* |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Glycoproteins; 10028-17-8/Tritium; 3713-31-3/Fucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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