| Extraction of extracellular polymeric substances from extreme acidic microbial biofilms. | |
| | |
MedLine Citation:
|
PMID: 18330567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The efficiency of five extraction methods for extracellular polymeric substances (EPS) was compared on three benthic eukaryotic biofilms isolated from an extreme acidic river, Río Tinto (SW, Spain). Three chemical methods (MilliQ water, NaCl, and ethylenediamine tetraacetic acid [EDTA]) and two physical methods (Dowex 50.8 and Crown Ether cation exchange resins) were tested. The quality and quantity of the EPS extracted from acidic biofilms varied according to which EPS extraction protocol was used. Higher amounts were obtained when NaCl and Crown Ether resins were used as extractant agents, followed by EDTA, Dowex, and MilliQ. EPS amounts varied from approximately 155 to 478 mg g(-1) of dry weight depending on the extraction method and biofilm analyzed. EPS were primarily composed of carbohydrate, heavy metals, and humic acid, plus small quantities of proteins and DNA. Neutral hexose concentrations corresponded to more than 90% of the total EPS dry weight. The proportions of each metals in the EPS extracted with EDTA are similar to the proportions present in the water from each locality where the biofilms were collected except for Al, Cu, Zn, and Pb. In this study, the extracellular matrix heavy metal sorption efficiencies of five methods for extracting EPS from eukaryotic acidic biofilms were compared. |
| | |
Authors:
|
Angeles Aguilera; Virginia Souza-Egipsy; Patxi San Martín-Uriz; Ricardo Amils |
Related Documents
:
|
10844557 - Microanalysis of melanins in mammalian hair by alkaline hydrogen peroxide degradation: ... 21790137 - Effect of hydrophilic organic seed aerosols on secondary organic aerosol formation from... 18961077 - Determination of phosphorus in hypereutectic aluminium-silicon alloys. 21059337 - Development of a microplate fluorescence assay for kynurenine aminotransferase. 6090027 - Unsaturated fatty acids stimulate nadph-dependent superoxide production by cell-free sy... 12660317 - Basolateral lat-2 has a major role in the transepithelial flux of l-cystine in the rena... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-11 |
Journal Detail:
|
Title: Applied microbiology and biotechnology Volume: 78 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-03-24 Completed Date: 2008-07-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
|
Languages: eng Pagination: 1079-88 Citation Subset: IM |
Affiliation:
|
Centro de Astrobiología (INTA-CSIC), Madrid, Spain. aguileraba@inta.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
chemistry*,
metabolism Biofilms / growth & development* Biomass Biopolymers / chemistry*, isolation & purification* Fresh Water / chemistry*, microbiology Microbiological Techniques* |
| Chemical | |
Reg. No./Substance:
|
0/Biopolymers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to incre...
Next Document: NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product d...