| Extraction and characterization of the insecticidal toxin hirsutellin A produced by Hirsutella thompsonii var. thompsonii. | |
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MedLine Citation:
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PMID: 8574903 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hirsutellin A (HtA) produced by Hirsutella thompsonii var. thompsonii (strain JAB-04) was extracted and purified using a combination of ion-exchange, gel-permeation, and immunoaffinity chromatography. The identity of the purified HtA was confirmed by amino acid analysis and N-terminal sequencing. Monoclonal antibodies prepared against HtA were capable of detecting 25-50 ng of HtA by direct sandwich ELISA. In addition, utilizing Western blot methods, the antibodies were shown to be specific to HtA. The production of HtA was monitored during submerged fermentation. The peak level of exocellular HtA (13-14 micrograms/ml) was during the late exponential growth phase (39-45 h), determined by utilizing a combination of densitometric analysis of the 16.3-kDa bands on SDS-PAGE gels and ELISA. HtA production was directly correlated with mycelial growth. Twenty-one-hour culture filtrates were highly toxic to larvae of the greater wax moth. Pure HtA at a final concentration of 40 pmol was highly toxic to Galleria mellonella larvae. |
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Authors:
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W Z Liu; D G Boucias; C W McCoy |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Experimental mycology Volume: 19 ISSN: 0147-5975 ISO Abbreviation: Exp. Mycol. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-03-12 Completed Date: 1996-03-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8511210 Medline TA: Exp Mycol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 254-62 Citation Subset: IM |
Affiliation:
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Entomology & Nematology Department, University of Florida, Gainesville 32611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Animals Antibodies, Monoclonal Chromatography, Affinity Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Fermentation Fungal Proteins / chemistry*, isolation & purification, toxicity Larva Lepidoptera Mitosporic Fungi / growth & development* Molecular Sequence Data Molecular Weight Peptide Fragments / chemistry, isolation & purification Pest Control, Biological* Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Antibodies, Monoclonal; 0/Fungal Proteins; 0/Peptide Fragments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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