Document Detail


Extraction and characterization of the insecticidal toxin hirsutellin A produced by Hirsutella thompsonii var. thompsonii.
MedLine Citation:
PMID:  8574903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hirsutellin A (HtA) produced by Hirsutella thompsonii var. thompsonii (strain JAB-04) was extracted and purified using a combination of ion-exchange, gel-permeation, and immunoaffinity chromatography. The identity of the purified HtA was confirmed by amino acid analysis and N-terminal sequencing. Monoclonal antibodies prepared against HtA were capable of detecting 25-50 ng of HtA by direct sandwich ELISA. In addition, utilizing Western blot methods, the antibodies were shown to be specific to HtA. The production of HtA was monitored during submerged fermentation. The peak level of exocellular HtA (13-14 micrograms/ml) was during the late exponential growth phase (39-45 h), determined by utilizing a combination of densitometric analysis of the 16.3-kDa bands on SDS-PAGE gels and ELISA. HtA production was directly correlated with mycelial growth. Twenty-one-hour culture filtrates were highly toxic to larvae of the greater wax moth. Pure HtA at a final concentration of 40 pmol was highly toxic to Galleria mellonella larvae.
Authors:
W Z Liu; D G Boucias; C W McCoy
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Experimental mycology     Volume:  19     ISSN:  0147-5975     ISO Abbreviation:  Exp. Mycol.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-03-12     Completed Date:  1996-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511210     Medline TA:  Exp Mycol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  254-62     Citation Subset:  IM    
Affiliation:
Entomology & Nematology Department, University of Florida, Gainesville 32611, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Animals
Antibodies, Monoclonal
Chromatography, Affinity
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Fermentation
Fungal Proteins / chemistry*,  isolation & purification,  toxicity
Larva
Lepidoptera
Mitosporic Fungi / growth & development*
Molecular Sequence Data
Molecular Weight
Peptide Fragments / chemistry,  isolation & purification
Pest Control, Biological*
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Antibodies, Monoclonal; 0/Fungal Proteins; 0/Peptide Fragments

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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