Document Detail

Extraction and characterisation of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus and Priacanthus macracanthus).
MedLine Citation:
PMID:  20355023     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Fish collagen has been paid increasing attention as an alternative to the mammalian counterpart owing to the abundance of fish skin as a processing by-product. Generally, the low yield of collagen extracted using the typical acid solubilisation process has led to the use of mammalian pepsin as an aid for increasing the yield. Alternatively, fish pepsin, especially from tuna stomach, can be used for the extraction of pepsin-solubilised collagen (PSC). Therefore the objective of this study was to extract and characterise PSC from the skin of bigeye snapper, a fish widely used for surimi production in Thailand. RESULTS: PSCs from the skin of two species of bigeye snapper, Priacanthus tayenus and Priacanthus macracanthus, were extracted with the aid of tongol tuna (Thunnus tonggol) pepsin and porcine pepsin. PSCs from the skin of both species extracted using porcine pepsin had a higher content of beta-chain but a lower content of alpha-chains compared with those extracted using tuna pepsin. All PSCs contained glycine as the major amino acid and had an imino acid (proline and hydroxyproline) content of 189-193 residues per 1000 residues. Transition temperatures of PSCs were in the range 30.6-31.3 degrees C. Fourier transform infrared spectra revealed some differences in molecular order between PSCs extracted using porcine pepsin and tuna pepsin. Nevertheless, the triple-helical structure of PSCs was not affected by pepsin digestion. Zeta potential analysis indicated that PSCs from P. tayens and P. macracanthus possessed zero net charge at pH 7.15-7.46 and 5.97-6.44 respectively. CONCLUSION: Tongol tuna pepsin could be used as a replacement for mammalian pepsin in PSC extraction. However, a slight difference in PSC properties was found.
Soottawat Benjakul; Yaowapa Thiansilakul; Wonnop Visessanguan; Sittiruk Roytrakul; Hideki Kishimura; Thummanoon Prodpran; Jirut Meesane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-03-31     Completed Date:  2010-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  132-8     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Society of Chemical Industry.
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
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MeSH Terms
Collagen / chemistry*,  isolation & purification*
Fish Proteins / chemistry*,  isolation & purification*,  metabolism
Glycine / analysis
Hydrogen-Ion Concentration
Imino Acids / analysis
Pepsin A / metabolism,  pharmacology
Perciformes* / metabolism
Protein Structure, Secondary
Skin / chemistry*,  metabolism
Spectroscopy, Fourier Transform Infrared
Reg. No./Substance:
0/Fish Proteins; 0/Imino Acids; 56-40-6/Glycine; 9007-34-5/Collagen; EC A

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