| Extraction and characterisation of hemicelluloses from maize stem. | |
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MedLine Citation:
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PMID: 20333614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Extraction and characterisation of hemicelluloses are very important for converting them into functional materials and chemicals. OBJECTIVE: To develop a method for isolation of hemicelluloses from all cell walls. METHODOLOGY: Sequential steps using 90% dioxane, 80% acidic dioxane, 100% dimethyl sulphoxide and 8% NaOH were used for extraction of the hemicellulosic preparations (H(1), H(2), H(3) and H(4)) from maize stem. Advanced NMR techniques were used for the analysis of native hemicelluloses. RESULTS: Hemicelluloses with high yieldd were isolated from all cell walls, and contained arabinoxylan as the major polysaccharide. H(3) was substituted by α-L-arabinofuranose, α-D-xylopyranose, and acetyl groups (degree of saturation = 0.12/0.09) at O-3/O-2 of xylan. H(4) had a long continuous side chain of arabinose residues, and associated closely with non-cellulosic glucose. The hemicelluloses formed more linkages with guaiacyl lignins, and some p-coumaric acids built a bridge between hemicelluloses and lignin in maize stem. CONCLUSION: This modified method is successful for the isolation of hemicelluloses with high yields from all cell walls of maize stem. |
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Authors:
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Xiao-Feng Sun; Paul Fowler; Mohanathas Rajaratnam; Guangcheng Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytochemical analysis : PCA Volume: 21 ISSN: 1099-1565 ISO Abbreviation: Phytochem Anal Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-10-08 Completed Date: 2011-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200492 Medline TA: Phytochem Anal Country: England |
Other Details:
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Languages: eng Pagination: 406-15 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Applied Chemistry, College of Science, Northwestern Polytechnical University, Xi'an, 710072, China. xf001sn@nwpu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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analysis Dimethyl Sulfoxide Dioxanes Hot Temperature Hydroxybenzoic Acids / analysis Lignin / isolation & purification Magnetic Resonance Spectroscopy Molecular Weight Plant Stems / chemistry Polysaccharides / chemistry*, isolation & purification Sodium Hydroxide Solvents Spectroscopy, Fourier Transform Infrared Thermodynamics Zea mays / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Dioxanes; 0/Hydroxybenzoic Acids; 0/Polysaccharides; 0/Solvents; 123-91-1/1,4-dioxane; 1310-73-2/Sodium Hydroxide; 29656-58-4/phenolic acid; 67-68-5/Dimethyl Sulfoxide; 8024-50-8/hemicellulose; 9005-53-2/Lignin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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