Document Detail

Extraction and characterisation of hemicelluloses from maize stem.
MedLine Citation:
PMID:  20333614     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Extraction and characterisation of hemicelluloses are very important for converting them into functional materials and chemicals.
OBJECTIVE: To develop a method for isolation of hemicelluloses from all cell walls.
METHODOLOGY: Sequential steps using 90% dioxane, 80% acidic dioxane, 100% dimethyl sulphoxide and 8% NaOH were used for extraction of the hemicellulosic preparations (H(1), H(2), H(3) and H(4)) from maize stem. Advanced NMR techniques were used for the analysis of native hemicelluloses.
RESULTS: Hemicelluloses with high yieldd were isolated from all cell walls, and contained arabinoxylan as the major polysaccharide. H(3) was substituted by α-L-arabinofuranose, α-D-xylopyranose, and acetyl groups (degree of saturation = 0.12/0.09) at O-3/O-2 of xylan. H(4) had a long continuous side chain of arabinose residues, and associated closely with non-cellulosic glucose. The hemicelluloses formed more linkages with guaiacyl lignins, and some p-coumaric acids built a bridge between hemicelluloses and lignin in maize stem.
CONCLUSION: This modified method is successful for the isolation of hemicelluloses with high yields from all cell walls of maize stem.
Xiao-Feng Sun; Paul Fowler; Mohanathas Rajaratnam; Guangcheng Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemical analysis : PCA     Volume:  21     ISSN:  1099-1565     ISO Abbreviation:  Phytochem Anal     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-10-08     Completed Date:  2011-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200492     Medline TA:  Phytochem Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  406-15     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Department of Applied Chemistry, College of Science, Northwestern Polytechnical University, Xi'an, 710072, China.
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MeSH Terms
Aldehydes / analysis
Dimethyl Sulfoxide
Hot Temperature
Hydroxybenzoic Acids / analysis
Lignin / isolation & purification
Magnetic Resonance Spectroscopy
Molecular Weight
Plant Stems / chemistry
Polysaccharides / chemistry*,  isolation & purification
Sodium Hydroxide
Spectroscopy, Fourier Transform Infrared
Zea mays / chemistry*
Reg. No./Substance:
0/Aldehydes; 0/Dioxanes; 0/Hydroxybenzoic Acids; 0/Polysaccharides; 0/Solvents; 123-91-1/1,4-dioxane; 1310-73-2/Sodium Hydroxide; 29656-58-4/phenolic acid; 67-68-5/Dimethyl Sulfoxide; 8024-50-8/hemicellulose; 9005-53-2/Lignin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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