Document Detail

Extraction of brown pigment from Rosa laevigata and its antioxidant activities.
MedLine Citation:
PMID:  21639686     Owner:  NLM     Status:  In-Data-Review    
Context: Rosa laevigata Michx. (Rosaceae), widespread in China, contains many valuable nutrients and has long been used as food and medicine in Chinese folklore. Nowadays, due to its favorable property of coloring, the brown pigment of R. laevigata has an attractive potential as an available additive in food. Objective: The aims of this study were to optimize the extraction process of brown pigment from R. laevigata and investigate its antioxidant activities on the basis of its abilities to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. Materials and methods: Extraction conditions of brown pigment from R. laevigata were investigated through an orthogonal design of L(9) (3)(4) assay. Ethanol concentration, extraction temperature, time, and ratio of material to solvent were the main factors affecting the extraction rate. Subsequently, the antioxidant activity of brown pigment was assessed using DPPH method, while hydroxyl radicals and superoxide free radicals were respectively determined by the Fenton-RhB (Rhodamine B) system and using the pyrogallol-luminol system. Results: The optimum extraction conditions were determined: temperature 70°C, ethanol concentration was 60%, extraction time 2 h and ratio of material to solvent was 1:6. Brown pigment showed a good radical scavenging activity, and exhibited a concentration-dependent inhibition of hydroxyl radical and superoxide free radical at low concentrations. When the concentration of brown pigment was 1 mg/mL, the scavenging percentage of hydroxyl radical reached 67.33%. Discussion and conclusion: The brown pigment of R. laevigata could potentially be used as a promising natural antioxidant in the food and pharmaceutical industries.
Kai-Jun Xiao; Xian-Ke Zhong; Jing Wang; Jian-Guo Jiang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmaceutical biology     Volume:  49     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  734-40     Citation Subset:  IM    
College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.
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