| Extraction of High-Quality RNA from Rubber Tree Leaves. | |
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MedLine Citation:
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PMID: 22785464 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE. |
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Authors:
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Liu-Hong Deng; Ming-Wu Luo; Chun-Fa Zhang; Hui-Cai Zeng |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: - ISSN: 1347-6947 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences. |
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