| Extraction and characterization of chitin, chitosan, and protein hydrolysates prepared from shrimp waste by treatment with crude protease from Bacillus cereus SV1. | |
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MedLine Citation:
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PMID: 19960271 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chitin is a polysaccharide found in abundance in the shell of crustaceans. In this study, the protease from Bacillus cereus SV1 was applied for chitin extraction from shrimp waste material of Metapenaeus monoceros. A high level of deproteinization 88.8% +/- 0.4 was recorded with an E/S ratio of 20. The demineralization was completely achieved within 6 h at room temperature in HCl 1.25 M, and the residual content of calcium in chitin was below 0.01%. (13)C CP/MAS-NMR spectral analysis of chitin prepared by the enzymatic deproteinization of shrimp wastes was found to be similar to that obtained by alkaline treatment and to the commercial alpha-chitin. The degree of N-acetylation, calculated from the spectrum, was 89.5%. Chitin obtained by treatment with crude protease from B. cereus was converted to chitosan by N-deacetylation, and the antibacterial activity of chitosan solution against different bacteria was investigated. Results showed that chitosan solution at 50 mg/mL markedly inhibited the growth of most Gram-negative and Gram-positive bacteria tested. Furthermore, the antioxidant potential of the protein hydrolysates obtained during enzymatic isolation of chitin was evaluated using various in vitro assays. All the samples exerted remarkable antioxidant activities. These results suggest that enzymatic deproteinization of the shrimp shell wastes, using B. cereus SV1 protease, could be applicable to the chitin production process. |
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Authors:
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Laila Manni; Olfa Ghorbel-Bellaaj; Kemel Jellouli; Islem Younes; Moncef Nasri |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-05 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 162 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-05-04 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 345-57 Citation Subset: IM |
Affiliation:
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Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, BP << W >> 3038 Sfax, Tunisia. lailouma@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Bacterial Agents / isolation & purification, metabolism, pharmacology Antioxidants / pharmacology Bacillus cereus / enzymology* Chitin / isolation & purification, metabolism* Chitosan / isolation & purification, metabolism*, pharmacology Crustacea / metabolism* Food Industry Gram-Negative Bacteria / drug effects Gram-Positive Bacteria / drug effects Industrial Waste Peptide Hydrolases / metabolism* Protein Hydrolysates / isolation & purification, metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Antioxidants; 0/Industrial Waste; 0/Protein Hydrolysates; 1398-61-4/Chitin; 9012-76-4/Chitosan; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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