Document Detail


Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)-magnetite nanoparticles.
MedLine Citation:
PMID:  22483894     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Although genetically modified (GM) food is becoming increasingly available, consumers are showing a growing awareness about the need to identify GM and non-GM foodstuff: the reliable identification of GM/non-GM food is therefore an important tool in the social, health and safety debates. The present research responds to this need (i) through developing a novel "single-pot" preparation of PAMAM magnetite nanoparticles (PMNPs) and by fully defining their specific characteristics; (ii) by demonstrating the capability of the PMNPs to isolate genomic DNA from different sample foods; and (iii) by experimentally demonstrating the identification of the isolated DNA by gel-electrophoresis, thus being capable of screening GM and non-GM food.
Authors:
Fengxiang Qie; Guoxin Zhang; Jianxuan Hou; Xiaoming Sun; Shi-Zhong Luo; Tianwei Tan
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Publication Detail:
Type:  Journal Article     Date:  2012-02-10
Journal Detail:
Title:  Talanta     Volume:  93     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  166-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
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