Document Detail

Extracellular trehalose utilization by Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16410039     Owner:  NLM     Status:  MEDLINE    
Two haploid strains of Saccharomyces cerevisiae viz. MATalpha and MATa were grown in glucose and trehalose medium and growth patterns were compared. Both strains show similar growth, except for an extended lag phase in trehalose grown cells. In both trehalose grown strains increase in activities of both extracellular trehalase activities and simultaneous decrease in extracellular trehalose level was seen. This coincided with a sharp increase in extracellular glucose level and beginning of log phase of growth. Alcohol production was also observed. Secreted trehalase activity was detected, in addition to periplasmic activity. It appeared that extracellular trehalose was hydrolyzed into glucose by extracellular trehalase activity. This glucose was utilized by the cells for growth. The alcohol formation was due to the fermentation of glucose. Addition of extracellular trehalase caused reduction in the lag phase when grown in trehalose medium, supporting our hypothesis of extracellular utilization of trehalose.
Arghya Basu; Soma Bhattacharyya; Paramita Chaudhuri; Subhabrata Sengupta; Anil K Ghosh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-27
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1760     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-06     Completed Date:  2006-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  134-40     Citation Subset:  IM    
Biotechnology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata 700032, India.
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MeSH Terms
Culture Media
Ethanol / metabolism
Glucose / metabolism
Hydrogen-Ion Concentration
Saccharomyces cerevisiae / growth & development,  metabolism*
Trehalase / metabolism,  secretion
Trehalose / metabolism*
Reg. No./Substance:
0/Culture Media; 50-99-7/Glucose; 64-17-5/Ethanol; 99-20-7/Trehalose; EC

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