Document Detail


Extracellular targeting of synthetic therapeutic nucleic acid formulations.
MedLine Citation:
PMID:  18855630     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Success of nucleic acid based therapies often depends on target-cell specific delivery of genetic materials such as plasmid DNA, antisense oligonucleotides or small interfering RNA. Such extracellular targeting strategies include the incorporation of hydrophilic shielding domains into nucleic acid carriers which protects them from unspecific interactions with non-target tissues (passive targeting), and the inclusion of targeting moieties which allows recognition of target-specific cellular receptors (active targeting). Furthermore physical targeting methods such as magnetofection, electroporation or by photochemical means have been used to enhance efficiency of nucleic acid transfer. For optimum efficacy, extracellular targeting concepts are combined with programmed bioresponsive carrier chemistry which confers to the formulation stability during extracellular delivery but controlled disassembly and nucleic acid release after reaching the target cell.
Authors:
Alexander Philipp; Martin Meyer; Ernst Wagner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current gene therapy     Volume:  8     ISSN:  1566-5232     ISO Abbreviation:  Curr Gene Ther     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-15     Completed Date:  2009-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125446     Medline TA:  Curr Gene Ther     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  324-34     Citation Subset:  IM    
Affiliation:
Pharmaceutical Biotechnology, Center for Drug Research, Department of Pharmacy, Ludwig-Maximilians-Universität, Butenandstr. 5-13, D-81377 Munich, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chemistry, Pharmaceutical
Drug Delivery Systems
Extracellular Space / genetics*
Gene Therapy / methods*
Hepatocytes / metabolism
Humans
Leukocytes / metabolism
Neoplasms / genetics,  therapy
Neurons / metabolism
Nucleic Acids / administration & dosage*,  genetics*
Photochemistry
Chemical
Reg. No./Substance:
0/Nucleic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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