| Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages. | |
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MedLine Citation:
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PMID: 20661257 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Macrophage colony-stimulating factor (M-CSF) (also known as CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have distinct effects on macrophage lineage populations, which are likely to be contributing to their functional heterogeneity. A comparative proteomic analysis of proteins released into culture media from such populations after M-CSF and GM-CSF exposure was carried out. Adherent macrophage populations, termed bone marrow-derived macrophage (BMM) and GM-BMM, were generated after treatment of murine bone marrow precursors with M-CSF and GM-CSF, respectively. Proteins in 16-h serum-free conditioned media (CM) were identified by two-dimensional gel electrophoresis and mass spectrometry. Respective protein profiles from BMM and GM-BMM CM were distinct and there was the suggestion of a switch from primarily signal peptide-driven secretion to non-classical secretion pathways from BMM to GM-BMM. Extracellular expression of cathepsins (lysosomal proteases) and their inhibitors seems to be a characteristic difference between these macrophage cell types with higher levels usually observed in BMM-CM. Furthermore, we have identified a number of proteins in BMM-CM and GM-BMM-CM that could be involved in various tissue regeneration and inflammatory (immune) processes, respectively. The uncharacterized protein C19orf10, a protein found at high levels in the synovial fluid of arthritis patients, was also differentially regulated; its extracellular levels were upregulated in the presence of GM-CSF. |
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Authors:
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Mark J Bailey; Derek C Lacey; Bernard Va de Kok; Paul D Veith; Eric C Reynolds; John A Hamilton |
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Publication Detail:
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Type: Journal Article Date: 2010-07-27 |
Journal Detail:
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Title: Immunology and cell biology Volume: 89 ISSN: 1440-1711 ISO Abbreviation: Immunol. Cell Biol. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8706300 Medline TA: Immunol Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 283-93 Citation Subset: IM |
Affiliation:
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Department of Medicine, CRC for Chronic Inflammatory Diseases, The University of Melbourne, The Royal Melbourne Hospital, Parkville, Victoria, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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