Document Detail


Extracellular protectants produced by Clostridium perfringens cells at elevated temperatures.
MedLine Citation:
PMID:  19055630     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS: Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, they were heat-shocked at 50 degrees C for 30 min. Then cultures were centrifuged and supernatants were transferred to nonshocked cells. Heat tolerance of these cells was performed at 55 degrees C. Viable cells were determined. In some cases, supernatants were heated at 65 degrees C or 100 degrees C or treated with trypsin. Supernatants were fractionated and PAGE was made of fractions showing heat-protective activity. When C. perfringens was exposed to a heat shock at 50 degrees C, extracellular factors were found in the culture supernatant that provided protection to cells not exposed to a heat shock. The extracellular factors were sensitive to heat and trypsin treatment suggesting a protein component. SDS-PAGE analysis of supernatant fractions from heat-treated cells revealed two induced proteins (56 and 125 kDa) that could be involved in heat tolerance. CONCLUSION: In this work, the presence and thermoprotective activity of extracellular factors produced by C. perfringens under a heat shock was demonstrated. SIGNIFICANCE AND IMPACT OF THE STUDY: The detection of thermoprotective extracellular factors of C. perfringens will aid in our understanding of the physiology of survival of C. perfringens in foods.
Authors:
N Heredia; P Ybarra; C Hernández; S García
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-02
Journal Detail:
Title:  Letters in applied microbiology     Volume:  48     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-02-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  133-9     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás, Nuevo León, México.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Bacterial Proteins / biosynthesis*,  chemistry
Clostridium perfringens / physiology*
Electrophoresis, Polyacrylamide Gel
Hot Temperature*
Microbial Viability
Molecular Weight
Chemical
Reg. No./Substance:
0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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