| Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E2 production in human A549 alveolar type II epithelial cells. | |
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MedLine Citation:
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PMID: 17481603 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Extracellular nucleotides regulate ion transport, mucociliary clearance as well as inflammatory properties of the airway epithelium by acting on P2 receptors. Cyclooxygenase-2 (COX-2) is a key enzyme involved in the synthesis of prostaglandins during inflammation. In this study, using calcium imaging, DNA microarray experiments, real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and prostaglandin E2 (PGE2) measurement, we show for the first time that ATP, UTP or INS365 compound (P2Y2 receptor agonists) up-regulate COX-2 expression by approximately 3-fold and enhance the release of PGE2 in human A549 airway epithelial cells. Our data suggest that P2Y receptors may represent putative targets in airway inflammatory diseases. |
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Authors:
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Brice Marcet; Frédérick Libert; Jean-Marie Boeynaems; Didier Communi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-14 |
Journal Detail:
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Title: European journal of pharmacology Volume: 566 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-05-28 Completed Date: 2007-09-19 Revised Date: 2010-01-14 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 167-71 Citation Subset: IM |
Affiliation:
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Institute of Interdisciplinary Research, IRIBHM, Université Libre de Bruxelles, and Department of Medical Chemistry, Erasme Hospital, Brussels, Belgium. marcet@ipmc.cnrs.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Cell Line Cyclooxygenase 2 / biosynthesis*, genetics Dinoprostone / biosynthesis* Epithelial Cells / drug effects*, metabolism Humans Inositol Phosphates / metabolism Nucleotides / pharmacology* Pulmonary Alveoli / cytology RNA, Messenger / metabolism Receptors, Purinergic P2 / agonists, genetics Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Nucleotides; 0/RNA, Messenger; 0/Receptors, Purinergic P2; 0/purinergic receptor P2Y2; 0/purinoceptor P2Y6; 363-24-6/Dinoprostone; 7440-70-2/Calcium; EC 1.14.99.1/Cyclooxygenase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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