Document Detail

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro.
MedLine Citation:
PMID:  18162537     Owner:  NLM     Status:  MEDLINE    
The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. In corals, three matrices are involved in spatial organization: (i) an organic ECM, which facilitates cell-cell and cell-substrate adhesion; (ii) a skeletal organic matrix (SOM), which facilitates controlled deposition of a calcium carbonate skeleton; and (iii) the calcium carbonate skeleton itself, which provides the structural support for the 3D organization of coral colonies. In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. In this system, which significantly facilitates studies of coral cell physiology, we demonstrate in vitro excretion of ECM by primary (nondividing) tissue cultures of both soft (Xenia elongata) and hard (Montipora digitata) corals. There are structural differences between the ECM produced by X. elongata cell cultures and that of M. digitata, and ascorbic acid, a critical cofactor for proline hydroxylation, significantly increased the production of collagen in the ECM of the latter species. We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata. Inductively coupled plasma mass spectrometry analysis of Sr/Ca ratios revealed the particles to be aragonite. De novo calcification was confirmed by following the incorporation of (45)Ca into acid labile macromolecules. Our results demonstrate the ability of isolated, differentiated coral cells to undergo fundamental processes required for multicellular organization.
Yael Helman; Frank Natale; Robert M Sherrell; Michèle Lavigne; Valentin Starovoytov; Maxim Y Gorbunov; Paul G Falkowski
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-27
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  105     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-09     Completed Date:  2008-02-05     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  54-8     Citation Subset:  IM    
Environmental Biophysics and Molecular Ecology Program and Inorganic Analytical Laboratory, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ 08901, USA.
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MeSH Terms
Agglutinins / chemistry
Calcification, Physiologic
Calcium Carbonate / chemistry*,  metabolism
Cell Survival
Cells, Cultured
Collagen / chemistry
Colorimetry / methods
DNA, Ribosomal / chemistry
Extracellular Matrix / metabolism*
Extracellular Matrix Proteins / chemistry
Lectins / chemistry
Microscopy, Electron, Scanning
Microscopy, Fluorescence / methods
Models, Biological
RNA, Ribosomal, 18S / chemistry
Triticum / metabolism
Reg. No./Substance:
0/Agglutinins; 0/DNA, Ribosomal; 0/Extracellular Matrix Proteins; 0/Lectins; 0/RNA, Ribosomal, 18S; 471-34-1/Calcium Carbonate; 9007-34-5/Collagen

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