Document Detail

Extracellular matrix metalloproteinase inducer/CD147 promotes myofibroblast differentiation by inducing alpha-smooth muscle actin expression and collagen gel contraction: implications in tissue remodeling.
MedLine Citation:
PMID:  17965264     Owner:  NLM     Status:  MEDLINE    
Extracellular matrix metalloproteinase inducer (EMMPRIN) is a cell surface glycoprotein enriched on tumor cells and normal epithelia. It is mainly known for its ability to induce matrix metalloproteinase production in fibroblasts following epithelial-stromal interaction. We sought to examine whether EMMPRIN has a broader role promoting fibroblast-to-myofibroblast differentiation. Because alpha-smooth muscle actin (alphaSMA) is considered a marker of this differentiation process, we analyzed the effect of EMMPRIN on its expression in corneal and skin fibroblasts by Western blots, immunocytochemistry, and a functional assay of collagen lattice contraction. Increasing EMMPRIN expression by cDNA transfection or by treatment with exogenously added recombinant EMMPRIN resulted in an up-regulation of alphaSMA expression. EMMPRIN also increased the contractile properties of the treated fibroblasts as demonstrated by the immunohistochemical appearance of stress fibers and by the accelerated contraction of fibroblast-embedded collagen lattices. Blocking EMMPRIN expression by small interfering RNA inhibited alphaSMA and collagen gel contraction induced not only by EMMPRIN but also by transforming growth factor-beta, a major mediator of myofibroblast differentiation that also regulated EMMPRIN expression. These findings, combined with the fact that EMMPRIN and alphaSMA colocalized to the same cells in the stroma of pathological corneas, expand on the mechanism by which EMMPRIN remodels extracellular matrix during wound healing and cancer.
Eric Huet; Benoit Vallée; Dominika Szul; Franck Verrecchia; Samia Mourah; James V Jester; Thanh Hoang-Xuan; Suzanne Menashi; Eric E Gabison
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-26
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  22     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-05-16     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1144-54     Citation Subset:  IM    
CRRET Laboratory, CNRS UMR 7149, University Paris XII, 61 Av du Général de Gaulle, 94010 Créteil Cedex, France.
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MeSH Terms
Actins / metabolism*
Antigens, CD147 / analysis,  metabolism*
Cell Differentiation
Cell Line
Collagen / physiology*
Corneal Stroma / metabolism
Fibroblasts / cytology*
Muscle Contraction
Muscle, Smooth / cytology,  metabolism*
Transforming Growth Factor beta / metabolism
Reg. No./Substance:
0/Actins; 0/Transforming Growth Factor beta; 136894-56-9/Antigens, CD147; 9007-34-5/Collagen

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