Document Detail


Extracellular matrix metalloproteinase inducer is increased in smokers' bronchoalveolar lavage fluid.
MedLine Citation:
PMID:  12714350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular matrix metalloproteinase inducer (EMMPRIN), also called basigin, is present in the lung during development, but its expression in normal adult lung is minimal. Increases of EMMPRIN have been found in various forms of experimental lung injury. To determine whether EMMPRIN might be involved in alveolar injury/repair associated with smoking, we developed an ELISA for EMMPRIN and applied it to bronchoalveolar lavage fluids from never-smokers (n = 7), former smokers (n = 16), and current smokers (n = 58). The smoker groups included subjects with emphysema, as determined by high-resolution chest computed tomography. EMMPRIN levels were significantly elevated in current and former smokers (315 +/- 20 and 175 +/- 15 pg/ml SEM, respectively, compared with 31 +/- 7 pg/ml in never-smokers), but the EMMPRIN levels of smokers with emphysema were not different from smokers without emphysema. Immunohistochemistry of smokers' lung tissue showed EMMPRIN in bronchiolar epithelium and alveolar macrophages, but EMMPRIN mRNA in alveolar macrophages was not different between current and never-smokers. Matrix metalloproteinase-1 was also detectable in the bronchoalveolar lavage fluid from some smokers but not in never-smokers. These findings indicate that smoking is associated with increased intrapulmonary EMMPRIN. Whether EMMPRIN is involved in smoking-induced lung pathology remains to be determined.
Authors:
Tomoko Betsuyaku; Mishie Tanino; Katsura Nagai; Yasuyuki Nasuhara; Masaharu Nishimura; Robert M Senior
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-04-24
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  168     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-09     Completed Date:  2003-08-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  222-7     Citation Subset:  AIM; IM    
Affiliation:
First Department of Medicine, Hokkaido University School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan. bytomoko@med.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antigens, CD*
Antigens, CD147
Antigens, Neoplasm*
Blotting, Western
Bronchoalveolar Lavage Fluid / chemistry*
Collagenases / metabolism*
Enzyme-Linked Immunosorbent Assay
Humans
Lung / metabolism,  pathology
Membrane Glycoproteins / metabolism*
Middle Aged
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Smoking / metabolism*
Grant Support
ID/Acronym/Agency:
HL29594/HL/NHLBI NIH HHS; HL47328/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Neoplasm; 0/BSG protein, human; 0/Membrane Glycoproteins; 0/RNA, Messenger; 136894-56-9/Antigens, CD147; EC 3.4.24.-/Collagenases

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