Document Detail


Extracellular matrix metalloproteinase inducer in interstitial pneumonias.
MedLine Citation:
PMID:  16867869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular matrix metalloproteinase inducer (EMMPRIN), a glycosylated transmembrane protein that induces matrix metalloproteinases (MMPs), is minimally expressed in the normal adult lung. We previously reported that it is up-regulated in murine bleomycin-induced lung injury. In this study, we determined the expression of EMMPRIN and its association with MMP-2, MMP-7, and MMP-9 in interstitial pneumonias (IPs). We performed immunohistochemistry for EMMPRIN and MMPs on lung tissue from 22 subjects with various IPs. We did bronchoalveolar lavage (BAL) on 9 of these subjects and 13 others with IPs to measure the soluble EMMPRIN in BAL fluid. For comparison, immunohistochemistry or BAL was done on 14 subjects without IPs. The staining intensity for each protein was scored from 0 to 3 in various epithelial cell types. Soluble EMMPRIN in BAL fluid was measured by an enzyme-linked immunosorbent assay. Extracellular matrix metalloproteinase inducer was prominent in abnormal epithelial cells. It was more prominent in hyperplastic type II cells, compared with epithelium in alveolar bronchiolization. It was also elevated in BAL fluid from the subjects with IPs. Matrix metalloproteinases were expressed in cells expressing EMMPRIN, although the profile of MMPs varied among the different abnormal epithelial cell types with MMP-2 and MMP-7 in hyperplastic type II cells and MMP-7 and MMP-9 in cells showing squamous metaplasia and cells comprising bronchiolization. These results suggest a role of EMMPRIN in reepithelialization in IPs.
Authors:
Nao Odajima; Tomoko Betsuyaku; Yasuyuki Nasuhara; Tomoo Itoh; Yuh Fukuda; Robert M Senior; Masaharu Nishimura
Related Documents :
2569019 - Effect of inhaled 239puo2 on alveolar type ii cells.
15074359 - Phenotype of regenerative epithelium in idiopathic interstitial pneumonias.
2251979 - Alveolar macrophage: origin, kinetics and relationship with cells of the alveolo-inters...
19484389 - An open-access microfluidic model for lung-specific functional studies at an air-liquid...
2272329 - In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
6494599 - Bronchoalveolar lavage in the normal lung. 2. cell distribution and cytomorphology.
15144579 - Glioma-specific and cell cycle-regulated herpes simplex virus type 1 amplicon viral vec...
18604869 - Ikappabalpha is required for marginal zone b cell lineage development.
18809459 - Matrix metalloproteinase-9 expression and release from skin fibroblasts interacting wit...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-06-02
Journal Detail:
Title:  Human pathology     Volume:  37     ISSN:  0046-8177     ISO Abbreviation:  Hum. Pathol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2006-09-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421547     Medline TA:  Hum Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1058-65     Citation Subset:  IM    
Affiliation:
First Department of Medicine, Hokkaido University Hospital, Sapporo 060-8638, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antigens, CD147 / metabolism*
Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid / chemistry
Epithelial Cells / metabolism,  pathology
Female
Humans
Immunohistochemistry
Lung / metabolism*,  pathology
Lung Diseases, Interstitial / metabolism*,  pathology
Male
Matrix Metalloproteinases / metabolism
Middle Aged
Grant Support
ID/Acronym/Agency:
HL47328/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
136894-56-9/Antigens, CD147; EC 3.4.24.-/Matrix Metalloproteinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The up-regulation profiles of p21WAF1/CIP1 and RUNX1/AML1 correlate with myometrial infiltration in ...
Next Document:  HIP/PAP, a member of the reg family, is expressed in glucagon-producing enteropancreatic endocrine c...