Document Detail


Extracellular matrix does not induce the proliferation, but promotes the differentiation, of Hodgkin's cell line HDLM-1.
MedLine Citation:
PMID:  3565539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tumor cells from the Hodgkin's cell line HDLM-1 were cultured in extracellular matrix (ECM)-coated flasks. Within 24 hours, the cells underwent rapid differentiation, accompanied by morphologic and phenotypic changes. A slow and less prominent, but similar change was observed in HDLM-1 cells after they had been induced with phorbol ester for 5 days. The ECM-induced cells became HeFi-1-negative and expressed antigens 2H9 and 1E9 on their nuclear membranes. These cells had punctate acid-phosphatase and esterase activities. Approximately 70% of the ECM-induced cells were elongated and had long, blunt cytoplasmic projections. These findings, together with evidence the authors previously presented, indicate that H-RS cells are related to interdigitating reticulum cells. The authors believe that the ECM-induced HDLM-1 cells can be used as an important model for studies of the nature and cell lineage of Hodgkin's disease.
Authors:
S M Hsu; X Zhao; P L Hsu; M S Lok
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of pathology     Volume:  127     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-05-13     Completed Date:  1987-05-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9-14     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / analysis
Antigens, Surface / analysis
Cell Adhesion
Cell Cycle
Cell Differentiation
Cell Division
Cells, Cultured
Extracellular Matrix / physiology*
Hodgkin Disease / immunology,  pathology*
Humans
Nuclear Envelope / immunology
Plastics
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Plastics
Comments/Corrections

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