| Extracellular matrix constituents and pigment cell expression in primary cell culture. | |
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MedLine Citation:
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PMID: 1287625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three types of pigment cells were isolated and cultured from larval Rana pipiens, and their attachment, maintenance, and proliferation were examined in the presence of extracellular matrix constituents (ECMs) in primary cell culture. The initial profile of pigment cell types present on day 2 of culture reflects the relative attachment of the cells to the dishes. Changes in the numbers of cells present after day 2 reflects the influence of factors present in the culture media on the maintenance, proliferation, or detachment of each type of pigment cell. Fetal bovine serum (FBS) promoted melanophore expression, but inhibited iridophore expression. FBS had no effect on xanthophores. In contrast, ventral skin conditioned medium (VCM), which contains melanization inhibiting factor, strongly stimulated iridophore expression, while it markedly inhibited melanophore expression. VCM had little effect on xanthophores. Of the ECMs tested, collagen type I had no effect on pigment cells. Fibronectin slightly inhibited melanophore expression, while it moderately stimulated iridophores and xanthophores. The stimulatory effect of fibronectin was not as strong as that of FBS or VCM. Laminin was also tested; however, it did not allow pigment cells to attach to the dishes, at least under the culture conditions utilized. The results of these experiments are discussed in terms of the general mechanisms of pigment pattern formation. |
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Authors:
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T Fukuzawa; S J Hamer; J T Bagnara |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society Volume: 5 ISSN: 0893-5785 ISO Abbreviation: Pigment Cell Res. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-03-15 Completed Date: 1993-03-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8800247 Medline TA: Pigment Cell Res Country: DENMARK |
Other Details:
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Languages: eng Pagination: 224-9 Citation Subset: IM |
Affiliation:
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Department of Anatomy, University of Arizona, Tucson 85724. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / drug effects Cell Division / drug effects Cells, Cultured / drug effects Culture Media, Conditioned / pharmacology Extracellular Matrix Proteins / pharmacology* Larva Melanophores / drug effects* Rana pipiens Skin |
| Grant Support | |
ID/Acronym/Agency:
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HD24445/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Extracellular Matrix Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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