Document Detail

Extracellular matrix components of Adipose Derived Stromal Cells promote alignment, organization and maturation of cardiomyocytes in vitro.
MedLine Citation:
PMID:  25111563     Owner:  NLM     Status:  Publisher    
Adipose Derived Stromal Cells (ADSC) are relevant therapeutic agents to treat myocardial infarction (MI) in clinical trials. Soluble factors secreted by ADSC, such as growth factors and cytokines, suppress inflammation and apoptosis while promoting angiogenesis and the proliferation of cardiomyocytes. Moreover, ADSC synthesize ECM components into the intercellular space which might contribute to their therapeutic capacity. Thus ADSC might directly modulate the post-MI microenvironment through a combination of paracrine and juxtacrine signaling. In this study the juxtacrine role of ADSC and ADSC-derived ECM on the organization and maturation of cardiomyocytes was investiagated. Human ADSC synthesized and deposited a heterogenous and complex mixture of ECM components such as collagen I, III, IV, fibronectin, elastin as well as the matricellular protein periostin. Co-cultures of rodent cardiomyocytes with human ADSC or with human ADSC-derived ECM components enhanced the cardiomyocyte alignment, their intercellular connections and the maturation of their sarcomeres, while the proliferation rate of the cardiomyocytes was increased and their level of hypertrophy reduced. The use of human ADSC-derived ECM could serve both to augment in vitro tissue-engineered myocardial constructs and to improve myocardial remodeling after infarction.
Ewa Przybyt; Marja J A van Luyn; Martin C Harmsen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-11
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Wiley Periodicals, Inc., A Wiley Company.
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