Document Detail


Extracellular lysosome-associated membrane protein-1 (LAMP-1) mediates autoimmune disease progression in the NOD model of type 1 diabetes.
MedLine Citation:
PMID:  15789355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment (from 5 to 25 weeks of age) with a novel blocking monoclonal antibody, mAb I-10, directed against the plasma membrane (pm) form of LAMP-1, protected against development of autoimmune diabetes in the NOD mouse. A shorter course of treatment, i.e. from 5 to 12 weeks of age, significantly reduced the occurrence of insulitis as well as disease onset. Interfering with pm-LAMP-1 required continuous treatment as tolerance was not observed when treatment was stopped, and no higher proportion of cells with a T regulatory phenotype (e.g. CD4(+)CD25(+)) were induced. The mechanism appears to involve modulating a proinflammatory cytokine, as the proportion of pancreatic-infiltrating IFN-gamma-positive cells was significantly reduced in the mAb I-10-treated group. These results demonstrate an unexpected role for pm-LAMP-1 in autoimmune disease progression, and suggest that further investigation should be performed to understand how this molecule modulates IFN-gamma-driven responses.
Authors:
Marcelo De Carvalho Bittencourt; Suzanne Herren; Pierre Graber; Francis Vilbois; Christian Pasquali; Claude Berney; Thomas Plitz; Ferdinando Nicoletti; Marie H Kosco-Vilbois
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  35     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-02     Completed Date:  2005-06-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1501-9     Citation Subset:  IM    
Affiliation:
Serono Pharmaceutical Research Institute, Geneva, Switzerland. marcelo.decarvalho@chu-fortefrance.fr
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Blocking / immunology*,  pharmacology
Antibodies, Monoclonal / immunology*,  pharmacology
Antigens, CD / immunology*
Blotting, Western
Diabetes Mellitus, Type 1 / immunology*,  prevention & control
Disease Models, Animal
Electrophoresis, Polyacrylamide Gel
Female
Flow Cytometry
Interferon-gamma / biosynthesis,  drug effects,  immunology
Lysosome-Associated Membrane Glycoproteins
Membrane Proteins / drug effects,  immunology*
Mice
Mice, Inbred NOD
Molecular Sequence Data
Pancreas / drug effects,  immunology
Th1 Cells / drug effects,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Blocking; 0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Proteins; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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