| Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein. | |
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MedLine Citation:
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PMID: 1576157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) secreted by Pseudomonas aeruginosa PAC1R was purified from cell-free growth medium by preparative isoelectric focusing. After blotting the N-terminal amino acid sequence and the amino acid composition were determined and compared to P. fragi and P. cepacia lipases yielding significant homology between all three species. Additionally, a consensus sequence K-Y-P-i-v-l-V-H-G was identified residing at the N-terminus of Pseudomonas lipases and in the central part of Staphylococcus lipases. Treatment of lipase with the serine-specific inhibitor diethyl p-nitrophenyl phosphate caused a rapid and complete inhibition of enzyme activity indicating the presence of a serine at the catalytic site as expected from lipase consensus sequences. Upon charge-shift electrophoresis the electrophoretic mobility of purified lipase was shifted either anodally or cathodally in the presence of sodium deoxycholate and cetyltrimethylammoniumbromide, respectively. This result demonstrates that extracellular lipase of P. aeruginosa exhibits an amphiphilic character like intrinsic membrane proteins. |
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Authors:
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K E Jaeger; F J Adrian; H E Meyer; R E Hancock; U K Winkler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1120 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-06-05 Completed Date: 1992-06-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 315-21 Citation Subset: IM |
Affiliation:
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Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität, Bochum, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Cholic Acids / pharmacology Detergents / pharmacology Isoelectric Focusing Lipase / antagonists & inhibitors, chemistry*, isolation & purification Molecular Sequence Data Phosphoric Acid Esters / pharmacology Protein Conformation Pseudomonas aeruginosa / enzymology* Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Cholic Acids; 0/Detergents; 0/Phosphoric Acid Esters; 598-02-7/diethyl phosphate; 75621-03-3/3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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